Anti-MED6 Antibody Picoband™

Product Name

Anti-MED6 Antibody Picoband™

View all MED6 Antibodies

SKU/Catalog Number

A09224

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MED6 Antibody Picoband™ catalog # A09224. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09224)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human MED6 recombinant protein (Position: F40-Q246).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09224 is reactive to MED6 in Human, Mouse, Rat

Applications

A09224 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

32 kDa

Calculated molecular weight

28.425kDa

Background of MED6

Mediator of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an enzyme that in humans is encoded by the MED6 gene. This family of proteins represent the transcriptional mediator protein subunit 6 that is required for activation of many RNA polymerase II promoters and which are conserved from yeast to humans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MED6 using anti-MED6 antibody (A09224).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: rat C6 whole cell lysates.
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED6 antigen affinity purified polyclonal antibody (Catalog # A09224) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED6 at approximately 34 kDa. The expected band size for MED6 is at 28 kDa.

Gene/Protein Information For MED6 (Source: Uniprot.org, NCBI)

Alternative Names

Activator-recruited cofactor 33 kDa component; ARC33; mediator complex subunit 6hMed6; mediator of RNA polymerase II transcription subunit 6; mediator of RNA polymerase II transcription, subunit 6 homolog (S. cerevisiae); mediator of RNA polymerase II transcription, subunit 6 homolog; NY-REN-28; Renal carcinoma antigen NY-REN-28 MED6 ARC33, NY-REN-28 mediator complex subunit 6 mediator of RNA polymerase II transcription subunit 6|CTD-2540L5.5|activator-recruited cofactor 33 kDa component|renal carcinoma antigen NY-REN-28

For more info on MED6, check out the MED6 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MED6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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