Anti-Mitofusin-2 MFN2 Antibody

Product Name

Anti-Mitofusin-2 MFN2 Antibody

View all Mitofusin 2 Antibodies

SKU/Catalog Number

A00461

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Mitofusin-2 MFN2 Antibody (Catalog # A00461). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MFN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Mitofusin-2 MFN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00461)

Host

Rabbit

Contents

MFN2 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

MFN2 antibody was raised against a 17 amino acid peptide near the center of human MFN2. The immunogen is located within amino acids 250 - 300 of MFN2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

MFN2 antibody is human, mouse and rat reactive. At least three isoforms of MFN2 are known to exist. MFN2 antibody is predicted to not cross-react with MFN1.

Reactive Species

A00461 is reactive to MFN2 in Human, Mouse, Rat

Applications

A00461 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

86.402kDa

Background of Mitofusin 2

Mitofusin 2 (MFN2) and the related protein MFN1 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN2 is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. MFN2 has two key domains: a coiled coil region that mediates MFN2 binding and a GTPase domain that likely plays a role in fusion (3,4). Both domains are essential for embryonic development and may play a role in the pathobiology of obesity. Overexpression of MFN2 causes mitochondrial dysfunction and cell death (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MFN2 antibody can be used for detection of MFN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of MFN2 in human brain tissue lysate with MFN2 antibody at 1 μg/ml.

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Immunohistochemistry of MFN2 in rat brain tissue with MFN2 antibody at 5 μg/mL.

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Immunofluorescence of MFN2 in rat brain tissue with MFN2 antibody at 20 μg/mL.

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Immunofluorescence of MFN2 in mouse brain tissue with MFN2 antibody at 20 μg/mL.

Green: MFN2 antibody (A00461)
Red: Phylloidin staining
Blue: DAPI staining

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Immunohistochemistry of MFN2 in mouse brain tissue with MFN2 antibody at 5 μg/mL.

Gene/Protein Information For MFN2 (Source: Uniprot.org, NCBI)

Alternative Names

CMT2A; CMT2A2; CPRP1; CPRP1mitochondrial assembly regulatory factor; EC 3.6.5; EC 3.6.5.-; HSG; KIAA0214hyperplasia suppressor; MARF; MFN2; Mitofusin 2; mitofusin-2; Transmembrane GTPase MFN2 MFN2 CMT2A, CMT2A2, CMT2A2A, CMT2A2B, CPRP1, HMSN6A, HSG, MARF mitofusin 2 mitofusin-2|hyperplasia suppressor|mitochondrial assembly regulatory factor|transmembrane GTPase MFN2

For more info on MFN2, check out the MFN2 Infographic

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In this infographic, you will see the following information for MFN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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