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Product Info Summary
| SKU: | A32468-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Mkl1/MRTFA Antibody Picoband™
SKU/Catalog Number
A32468-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mkl1/MRTFA Antibody Picoband™ catalog # A32468-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Mkl1/MRTFA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32468-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mkl1/MRTFA recombinant protein (Position: L192-L931).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A32468-2 is reactive to MRTFA in Human
Applications
A32468-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
145 kDa
Calculated molecular weight
98.919kDa
Background of Mkl1
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Mkl1/MRTFA using anti-Mkl1/MRTFA antibody (A32468-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mkl1/MRTFA antigen affinity purified polyclonal antibody (Catalog # A32468-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mkl1/MRTFA at approximately 145 kDa. The expected band size for Mkl1/MRTFA is at 145 kDa.
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Figure 2. IF analysis of Mkl1/MRTFA using anti-Mkl1/MRTFA antibody (A32468-2).
Mkl1/MRTFA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Mkl1/MRTFA Antibody (A32468-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For MRTFA (Source: Uniprot.org, NCBI)
Gene Name
MRTFA
Full Name
Myocardin-related transcription factor A
Weight
98.919kDa
Alternative Names
AMKL; KIAA1438; MALSAP and coiled-coil domain; megakaryoblastic leukemia (translocation) 1; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; MKL/myocardin-like protein 1; Myocardin-related transcription factor A; RNA-binding motif protein 15/megakaryoblastic leukemia-1 fusion protein MRTFA BSAC, MAL, MKL, MKL1, MRTF-A myocardin related transcription factor A myocardin-related transcription factor A|MKL/myocardin-like protein 1|basic, SAP and coiled-coil domain|megakaryoblastic leukemia (translocation) 1|megakaryoblastic leukemia 1 protein|megakaryocytic acute leukemia protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRTFA, check out the MRTFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRTFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mkl1/MRTFA Antibody Picoband™ (A32468-2)
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No publications found for A32468-2
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