Product Info Summary
SKU: | A00733-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MMP1 Antibody Picoband™
SKU/Catalog Number
A00733-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MMP1 Antibody Picoband™ catalog # A00733-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00733-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MMP1 recombinant protein (Position: D124-N469).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00733-1 is reactive to MMP1 in Human
Applications
A00733-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.007kDa
Background of MMP-1
Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase, is an enzyme that in humans is encoded by the MMP1 gene. MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. It is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MMP1 using anti-MMP1 antibody (A00733-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (Catalog # A00733-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP1 at approximately 54 kDa. The expected band size for MMP1 is at 54 kDa.
Click image to see more details
Figure 2. IHC analysis of MMP1 using anti-MMP1 antibody (A00733-1).
MMP1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MMP1 using anti-MMP1 antibody (A00733-1).
MMP1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of MMP1 using anti-MMP1 antibody (A00733-1).
MMP1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of MMP1 using anti-MMP1 antibody (A00733-1).
MMP1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of PC-3 cells using anti-MMP1 antibody (A00733-1).
Overlay histogram showing PC-3 cells stained with A00733-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMP1 Antibody (A00733-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MMP1 (Source: Uniprot.org, NCBI)
Gene Name
MMP1
Full Name
Interstitial collagenase
Weight
54.007kDa
Superfamily
peptidase M10A family
Alternative Names
CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1 MMP1 CLG, CLGN matrix metallopeptidase 1 interstitial collagenase|fibroblast collagenase|matrix metallopeptidase 1 (interstitial collagenase)|matrix metalloprotease 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP1, check out the MMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP1 Antibody Picoband™ (A00733-1)
Hello CJ!
A00733-1 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia
IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation
Chu X,Yu T,Huang X,Xi Y,Ni B,Zhang R,You H.Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats.Aging (Albany NY).2020 Jul 5;12(13):12799-12811.doi:10.18632/aging.103222.Epub 2020
Species: Rat
A00733-1 usage in article: APP:WB, SAMPLE:PRIMARY CHONDROCYTES, DILUTION:1:1000
Xie LL,Zhao YL,Yang J,Cheng H,Zhong ZD,Liu YR,Pang XL.Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats.Biomed Res Int.2020 Jul 6;2020:9380965.doi:10.1155/2020/9380965.PMID: 32724821;PMCID:PMC7366230.
Species: Rat
A00733-1 usage in article: APP:IHC, SAMPLE:JOINT TISSUE, DILUTION:1:100
High plasma pentraxin 3 levels in diabetic polyneuropathy patients with nociceptive pain
Andrographolide plays an important role in bleomycin-induced pulmonary fibrosis treatment
Deng Cl, Ling St, Liu Xq, Zhao Yj, Lv Yf. Exp Ther Med. 2015 Mar;9(3):992-998. Epub 2015 Jan 20. Decreased Expression Of Matrix Metalloproteinase-1 In The Maternal Umbilical Serum, Trophoblasts And Decidua Leads To Preeclampsia.
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-MMP1 Antibody Picoband™
Question
We ordered your anti-MMP1 antibody for ELISA on synovial cell in the past. I am using human, and We intend to use the antibody for IHC-P next. We want examining synovial cell as well as smooth muscle tissue in our next experiment. Do you have any suggestion on which antibody would work the best for IHC-P?
D. Jones
Verified customer
Asked: 2019-10-10
Answer
I took a look at the website and datasheets of our anti-MMP1 antibody and it appears that A00733-1 has been validated on human in both ELISA and IHC-P. Thus A00733-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-MMP1 antibody A00733-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The anti-MMP1 antibody (A00733-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-07
Question
We have observed staining in human ovary. Any tips? Is anti-MMP1 antibody supposed to stain ovary positively?
Verified Customer
Verified customer
Asked: 2017-09-15
Answer
From what I have seen in literature ovary does express MMP1. From what I have seen in Uniprot.org, MMP1 is expressed in smooth muscle tissue, ovary, synovial cell, fibroblast, among other tissues. Regarding which tissues have MMP1 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2557822
Ovary, Pubmed ID: 15489334
Synovial cell, Pubmed ID: 3027129
Boster Scientific Support
Answered: 2017-09-15
Question
My boss were well pleased with the WB result of your anti-MMP1 antibody. However we have seen positive staining in smooth muscle tissue extracellular using this antibody. Is that expected? Could you tell me where is MMP1 supposed to be expressed?
L. Anderson
Verified customer
Asked: 2014-09-29
Answer
From literature, smooth muscle tissue does express MMP1. Generally MMP1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP1 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2557822
Ovary, Pubmed ID: 15489334
Synovial cell, Pubmed ID: 3027129
Boster Scientific Support
Answered: 2014-09-29
Question
We need using your anti-MMP1 antibody for interleukin-4 and interleukin-13 signaling studies. Has this antibody been tested with western blotting on u2os whole cell lysates? We would like to see some validation images before ordering.
F. Bhatt
Verified customer
Asked: 2014-03-31
Answer
Thank you for your inquiry. This A00733-1 anti-MMP1 antibody is validated on human k562, k562 whole cell lysates, u2os whole cell lysates. It is guaranteed to work for ELISA, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-03-31