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Product Info Summary
| SKU: | M00344-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP |
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Product info
Product Name
Anti-Ms CD4 Purified Azide Free Monoclonal Antibody
SKU/Catalog Number
M00344-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD4 Purified Azide Free Monoclonal Antibody (Catalog# M00344-3). Tested in Flow Cytometry, IP application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD4 Purified Azide Free Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00344-3)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, azide-free
Clonality
Monoclonal
Clone Number
BB7.2
Isotype
Mouse IgG2b
Immunogen
HLA-A2 solubilised by papain. The antibody BB7.2 recognizes an extracellular epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands within the human HLA-A2 histocompatibility antigen.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00344-3 is reactive to CD4 in Human
Applications
M00344-3 is guaranteed for Flow Cytometry, IP Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
51.111kDa
Background of CD4
HLA-A2 (44 kDa) is the most frequent HLA-A allele in human ethnic populations. HLA-A, together with HLA-B and HLA-C, represent human HLA class I major histocompatibility (MHC) antigens. These intrinsic membrane glycoproteins are expressed on nucleated cells and noncovalently associate with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of Cd4 using Anti-Ms CD4 Purified Azide Free Monoclonal Antibody (M00344-3).
Flow cytometry analysis (surface staining) of CD4 in murine splenocytes with anti-CD4 (GK1.5) azide free
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Figure 2. Flow Cytometry validation of Cd4 using Anti-Ms CD4 Purified Azide Free Monoclonal Antibody (M00344-3).
Separation of murine CD4 positive cells (red-filled) from murine CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (azide free
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Figure 3. Flow Cytometry validation of Cd4 using Anti-Ms CD4 Purified Azide Free Monoclonal Antibody (M00344-3).
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (azide freeProtein Target Info & Infographic
Gene/Protein Information For CD4 (Source: Uniprot.org, NCBI)
Gene Name
CD4
Full Name
T-cell surface glycoprotein CD4
Weight
51.111kDa
Alternative Names
CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 CD4 CD4mut CD4 molecule T-cell surface glycoprotein CD4|CD4 antigen (p55)|CD4 receptor|T-cell surface antigen T4/Leu-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD4, check out the CD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ms CD4 Purified Azide Free Monoclonal Antibody (M00344-3)
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No publications found for M00344-3
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