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Product Info Summary
| SKU: | M00001-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Mutant p53 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00001-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Mutant p53 Rabbit Monoclonal Antibody catalog # M00001-3. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mutant p53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00001-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Mutant p53
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00001-3 is reactive to TP53 in Human
Applications
M00001-3 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
43.653kDa
Background of p53
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of p53 in (1) A431 cell lysate; (2) HEK293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)
Gene Name
TP53
Full Name
Cellular tumor antigen p53
Weight
43.653kDa
Superfamily
p53 family
Alternative Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mutant p53 Rabbit Monoclonal Antibody (M00001-3)
Hello CJ!
M00001-3 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).
Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.
Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Mutant p53 Rabbit Monoclonal Antibody
Question
Can M00001-3 be used for IHC?
Verified customer
Asked: 2022-06-28
Answer
The Anti-Mutant P53 Rabbit Monoclonal Antibody (M00001-3) can be used for IHC human tissue.
Boster Scientific Support
Answered: 2022-06-28
Question
We have tried in the past anti-Mutant p53 Rabbit Monoclonal antibody for IP on embryonic kidney a few months ago. I am using human, and We intend to use the antibody for WB next. My question regards examining embryonic kidney as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
K. Moore
Verified customer
Asked: 2020-04-16
Answer
I looked at the website and datasheets of our anti-Mutant p53 Rabbit Monoclonal antibody and I see that M00001-3 has been tested on human in both IP and WB. Thus M00001-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung carcinoma using anti-Mutant p53 Rabbit Monoclonal antibody M00001-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-31
Question
I was wanting to use your anti-Mutant p53 Rabbit Monoclonal antibody for IF for human lung carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
You can see on the product datasheet, M00001-3 anti-Mutant p53 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-27
Question
Would M00001-3 anti-Mutant p53 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can see on the product datasheet, M00001-3 anti-Mutant p53 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-15
Question
My question regarding product M00001-3, anti-Mutant p53 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00001-3 anti-Mutant p53 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-28
Question
Do you have a BSA free version of anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 available?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-27
Question
My colleagues were content with the WB result of your anti-Mutant p53 Rabbit Monoclonal antibody. However we have been able to see positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
From literature, kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-06-12
Question
I am interested in using your anti-Mutant p53 Rabbit Monoclonal antibody for cardiac septum morphogenesis studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
Thank you for your inquiry. This M00001-3 anti-Mutant p53 Rabbit Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-21
Question
We have observed staining in human glial cell glial tumor. Do you have any suggestions? Is anti-Mutant p53 Rabbit Monoclonal antibody supposed to stain glial cell glial tumor positively?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
Based on literature glial cell glial tumor does express TP53. Based on Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-02-05
Question
Is a blocking peptide available for product anti-Mutant p53 Rabbit Monoclonal antibody (M00001-3)?
R. Zhao
Verified customer
Asked: 2019-01-29
Answer
We do provide the blocking peptide for product anti-Mutant p53 Rabbit Monoclonal antibody (M00001-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-29
Question
Does anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 work for IF with lung carcinoma?
M. Zhao
Verified customer
Asked: 2019-01-18
Answer
According to the expression profile of lung carcinoma, TP53 is highly expressed in lung carcinoma. So, it is likely that anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 will work for IF with lung carcinoma.
Boster Scientific Support
Answered: 2019-01-18
Question
We need to test anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 on human lung carcinoma for research purposes, then I may be interested in using anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
The products we sell, including anti-Mutant p53 Rabbit Monoclonal antibody M00001-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-07
Question
I have attached the WB image, lot number and protocol we used for lung carcinoma using anti-Mutant p53 Rabbit Monoclonal antibody M00001-3. Please let me know if you require anything else.
R. Evans
Verified customer
Asked: 2018-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-17
Question
Is this M00001-3 anti-Mutant p53 Rabbit Monoclonal antibody reactive to the isotypes of TP53?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
The immunogen of M00001-3 anti-Mutant p53 Rabbit Monoclonal antibody is A synthesized peptide derived from human Mutant p53. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-09
Question
I see that the anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 works with IF, what is the protocol used to produce the result images on the product page?
J. Gonzalez
Verified customer
Asked: 2016-08-02
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-08-02
Question
We are currently using anti-Mutant p53 Rabbit Monoclonal antibody M00001-3 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
G. Parker
Verified customer
Asked: 2013-09-16
Answer
The anti-Mutant p53 Rabbit Monoclonal antibody (M00001-3) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-16
