Product Info Summary
SKU: | A01697-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MYLK Antibody
View all Myosin light chain kinase Antibodies
SKU/Catalog Number
A01697-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MYLK Antibody catalog # A01697-1. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MYLK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01697-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Cot around the phosphorylation site of T290.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01697-1 is reactive to MYLK in Human, Mouse, Rat
Applications
A01697-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
210.715kDa
Background of Myosin light chain kinase
Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development By similarity. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.
Lucka L., FEBS Lett. 454:341-344(1999).
Seppala R., Am. J. Hum. Genet. 64:1563-1569(1999).
Huizing M., Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2,000-1:10,000
ICC: 1:100-1:500
IHC: 1:100-1:500
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of MYLK using Anti-MYLK Antibody (A01697-1).
ICC staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-MYLK Antibody A01697-1
Western blot analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5Protein Target Info & Infographic
Gene/Protein Information For MYLK (Source: Uniprot.org, NCBI)
Gene Name
MYLK
Full Name
Myosin light chain kinase, smooth muscle
Weight
210.715kDa
Superfamily
protein kinase superfamily
Alternative Names
DKFZp686I10125; EC 2.7.11; EC 2.7.11.18; FLJ12216; Kinase-related protein; KRP; light polypeptide kinase; MLCK1; MLCK108; MLCKMLCK210; MSTP083; MYLK1; myosin light chain kinase; smooth muscle myosin light chain kinase; smooth muscle; telokin MYLK AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MMIHS, MSTP0831, smMLCK, MYLK myosin light chain kinase myosin light chain kinase, smooth muscle|kinase-related protein|myosin, light polypeptide kinase|smooth muscle myosin light chain kinase|telokin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYLK, check out the MYLK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYLK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYLK Antibody (A01697-1)
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No publications found for A01697-1
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Customer Reviews
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