Anti-N-Cadherin-2 CDH2 CD325-Antibody

N-Cadherin antibody

Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Antibody catalog # PA1328. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1328
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, WB

Product Name

Anti-N-Cadherin-2 CDH2 CD325-Antibody

View all N-Cadherin Antibodies

SKU/Catalog Number

PA1328

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Antibody catalog # PA1328. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-N-Cadherin-2 CDH2 CD325-Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1328)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1328 is reactive to CDH2 in Human, Mouse, Rat

Applications

PA1328 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

140 kDa

Calculated molecular weight

99.809kDa

Background of N-Cadherin

N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Human, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDH2 (Source: Uniprot.org, NCBI)

Gene Name

CDH2

Full Name

Cadherin-2

Weight

99.809kDa

Alternative Names

cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin CDH2 ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD cadherin 2 cadherin-2|N-cadherin 1|cadherin 2, type 1, N-cadherin (neuronal)|calcium-dependent adhesion protein, neuronal|neural cadherin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDH2, check out the CDH2 Infographic

CDH2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1328 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
PA1328 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200

Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
PA1328 usage in article: APP:IF, SAMPLE:BREAST CANCER CELLS AND BREAST TISSUE, DILUTION:1:100

Xu H,Yu B,Shen W,Jin C,Wang L,Xi Y.Over-expression of long non-coding RNA ZEB2-AS1 may predict poor prognosis and promote the migration, invasion, and epithelial-mesenchymal transition of tumor cells in non-small cell lung cancer.Int J Biol Markers.2020 S
Species: Human
PA1328 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:1000

Jia X, Wang H,Li Z,Yan J,Guo Y,Zhao W,Gao L,Wang B,Jia Y.HER4 promotes the progression of colorectal cancer by promoting epithelial‑mesenchymal transition.Mol Med Rep.2020 Apr;21(4):1779-1788.doi:10. 3892/mmr.2020.10974.Epub 2020 Feb 4.PMID:32319604;PMCID
Species: Human

Lu N,Piao MH,Feng CS,Yuan Y.Isoflurane promotes epithelial-to-mesenchymal transition and metastasis of bladder cancer cells through HIF-1α-β-catenin/Notch1 pathways. Life Sci. 2020 Oct 1;258:118154.doi: 10.1016/j.lfs.2020.118154.Epub 2020 Jul 28.PMID: 327
Species: Human,Mouse
PA1328 usage in article: APP:WB, SAMPLE:T24 CELLS AND BIU-87 CELLS, DILUTION:1:100

Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
PA1328 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA

Expression of N%u2011cadherin proteins in myocardial hypertrophy in rats

Expression of N-cadherin in myocardial tissues during the development of a rat heart

Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-beta%u2013mediated epithelial%u2013mesenchymal transition

Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy

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7 Customer Q&As for Anti-N-Cadherin-2 CDH2 CD325-Antibody

Question

We are currently using anti-N Cadherin/CDH2 antibody PA1328 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

The anti-N Cadherin/CDH2 antibody (PA1328) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-06

Question

I was wanting to use your anti-N Cadherin/CDH2 antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

You can see on the product datasheet, PA1328 anti-N Cadherin/CDH2 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-25

Question

I see that the anti-N Cadherin/CDH2 antibody PA1328 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-14

Question

I have a question about product PA1328, anti-N Cadherin/CDH2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Singh

Verified customer

Asked: 2019-02-04

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1328 anti-N Cadherin/CDH2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-02-04

Question

Would anti-N Cadherin/CDH2 antibody PA1328 work on bovine IHC with liver?

Verified Customer

Verified customer

Asked: 2017-12-15

Answer

Our lab technicians have not tested anti-N Cadherin/CDH2 antibody PA1328 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-N Cadherin/CDH2 antibody PA1328 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-15

Question

you antibody to test anti-N Cadherin/CDH2 antibody PA1328 on rat testis for research purposes, then I may be interested in using anti-N Cadherin/CDH2 antibody PA1328 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Zhang

Verified customer

Asked: 2015-07-30

Answer

The products we sell, including anti-N Cadherin/CDH2 antibody PA1328, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-07-30

Question

Is a blocking peptide available for product anti-N Cadherin/CDH2 antibody (PA1328)?

A. Brown

Verified customer

Asked: 2015-01-07

Answer

We do provide the blocking peptide for product anti-N Cadherin/CDH2 antibody (PA1328). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-01-07

Order DetailsPrice
PA1328

100μg

$370
PA1328-10ug

10μg sample (liquid)

$99
PA1328-Biotin

100 μg Biotin conjugated

$570
PA1328-Cy3

100 μg Cy3 conjugated

$570
PA1328-Dylight488

100 μg Dylight488 conjugated

$570
PA1328-Dylight550

100 μg Dylight550 conjugated

$570
PA1328-Dylight594

100 μg Dylight594 conjugated

$570
PA1328-FITC

100 μg FITC conjugated

$570
PA1328-HRP

100 μg HRP conjugated

$570
PA1328-APC

100 μg APC conjugated

$670
PA1328-PE

100 μg PE conjugated

$670
PA1328-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1328
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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