Product Info Summary
SKU: | PA1328 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-N-Cadherin-2 CDH2 CD325-Antibody
View all N-Cadherin Antibodies
SKU/Catalog Number
PA1328
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Antibody catalog # PA1328. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-N-Cadherin-2 CDH2 CD325-Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1328)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1328 is reactive to CDH2 in Human, Mouse, Rat
Applications
PA1328 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
140 kDa
Calculated molecular weight
99.809kDa
Background of N-Cadherin
N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Human, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CDH2 using anti-CDH2 antibody (PA1328).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: human SKOV3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # PA1328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140KD. The expected band size for CDH2 is at 100KD.
Click image to see more details
Figure 2. IHC analysis of CDH2 using anti-CDH2 antibody (PA1328).
CDH2 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CDH2 using anti-CDH2 antibody (PA1328).
CDH2 was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CDH2 using anti-CDH2 antibody (PA1328).
CDH2 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of Hela cells using anti-CDH2 antibody (PA1328).
Overlay histogram showing Hela cells stained with PA1328 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDH2 Antibody (PA1328, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 6. IF analysis of CDH2 using anti-CDH2 antibody (PA1328).
CDH2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. IF analysis of CDH2 using anti-CDH2 antibody (PA1328).
CDH2 was detected in a paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CDH2 (Source: Uniprot.org, NCBI)
Gene Name
CDH2
Full Name
Cadherin-2
Weight
99.809kDa
Alternative Names
cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin CDH2 ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD cadherin 2 cadherin-2|N-cadherin 1|cadherin 2, type 1, N-cadherin (neuronal)|calcium-dependent adhesion protein, neuronal|neural cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH2, check out the CDH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-N-Cadherin-2 CDH2 CD325-Antibody (PA1328)
Hello CJ!
PA1328 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
PA1328 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
PA1328 usage in article: APP:IF, SAMPLE:BREAST CANCER CELLS AND BREAST TISSUE, DILUTION:1:100
Xu H,Yu B,Shen W,Jin C,Wang L,Xi Y.Over-expression of long non-coding RNA ZEB2-AS1 may predict poor prognosis and promote the migration, invasion, and epithelial-mesenchymal transition of tumor cells in non-small cell lung cancer.Int J Biol Markers.2020 S
Species: Human
PA1328 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:1000
Jia X, Wang H,Li Z,Yan J,Guo Y,Zhao W,Gao L,Wang B,Jia Y.HER4 promotes the progression of colorectal cancer by promoting epithelial‑mesenchymal transition.Mol Med Rep.2020 Apr;21(4):1779-1788.doi:10. 3892/mmr.2020.10974.Epub 2020 Feb 4.PMID:32319604;PMCID
Species: Human
Lu N,Piao MH,Feng CS,Yuan Y.Isoflurane promotes epithelial-to-mesenchymal transition and metastasis of bladder cancer cells through HIF-1α-β-catenin/Notch1 pathways. Life Sci. 2020 Oct 1;258:118154.doi: 10.1016/j.lfs.2020.118154.Epub 2020 Jul 28.PMID: 327
Species: Human,Mouse
PA1328 usage in article: APP:WB, SAMPLE:T24 CELLS AND BIU-87 CELLS, DILUTION:1:100
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
PA1328 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA
Expression of N%u2011cadherin proteins in myocardial hypertrophy in rats
Expression of N-cadherin in myocardial tissues during the development of a rat heart
Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-beta%u2013mediated epithelial%u2013mesenchymal transition
Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-N-Cadherin-2 CDH2 CD325-Antibody
Question
We are currently using anti-N Cadherin/CDH2 antibody PA1328 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The anti-N Cadherin/CDH2 antibody (PA1328) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-06
Question
I was wanting to use your anti-N Cadherin/CDH2 antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
You can see on the product datasheet, PA1328 anti-N Cadherin/CDH2 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-25
Question
I see that the anti-N Cadherin/CDH2 antibody PA1328 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-14
Question
I have a question about product PA1328, anti-N Cadherin/CDH2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Singh
Verified customer
Asked: 2019-02-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1328 anti-N Cadherin/CDH2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-04
Question
Would anti-N Cadherin/CDH2 antibody PA1328 work on bovine IHC with liver?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
Our lab technicians have not tested anti-N Cadherin/CDH2 antibody PA1328 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-N Cadherin/CDH2 antibody PA1328 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-15
Question
you antibody to test anti-N Cadherin/CDH2 antibody PA1328 on rat testis for research purposes, then I may be interested in using anti-N Cadherin/CDH2 antibody PA1328 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Zhang
Verified customer
Asked: 2015-07-30
Answer
The products we sell, including anti-N Cadherin/CDH2 antibody PA1328, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-07-30
Question
Is a blocking peptide available for product anti-N Cadherin/CDH2 antibody (PA1328)?
A. Brown
Verified customer
Asked: 2015-01-07
Answer
We do provide the blocking peptide for product anti-N Cadherin/CDH2 antibody (PA1328). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-01-07