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CDH2 antibody (PA1328) performs well in Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 53 Publications

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pa1328 cdh2 primary antibodies fcm testing 1

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Flow Cytometry analysis of A549 cells using anti-CDH2 antibody (PA1328).
Overlay histogram showing A549 cells stained with PA1328 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CDH2 Antibody (PA1328, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband®

CDH2 antibody

53 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, WB

Tested Applications

CDH2 antibody was positively detected by WB in:

human 293T whole cell, human Hela whole cell, rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CDH2 antibody was positively detected by IHC in:

mouse heart tissue, rat heart tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CDH2 antibody was positively detected by IF in:

rat heart tissue

CDH2 antibody was positively detected by FC in:

A549 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Mouse

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband®
View all CDH2 Antibodies

Form

Lyophilized

Uniprot

P19022

Gene ID

1000

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin, identical to the related rat and mouse sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

140 kDa

Calculated Molecular Weight

99.8 kDa

Background of CDH2

N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Cite This Product

Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1328)

Protocols

Western Blot protocol for CDH2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CDH2 antibody

Review Protocol

Immunofluorescence (IF) protocol for CDH2 antibody

Review Protocol

Flow Cytometry (FC) protocol for CDH2 antibody

Review Protocol

Publications

CBLC-mediated ubiquitination stabilizes METTL1, enhances N7-methylguanosine modification of ESRRA, and promotes the progression of endometrial cancer

Published: 2026-05-30

Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Application: WB

Impact Factor: 8.70

Species: Human

PMID: 42217705

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Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband®

CDH2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

CBLC-mediated ubiquitination stabilizes METTL1, enhances N7-methylguanosine modification of ESRRA, and promotes the progression of endometrial cancer

STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT

Geniposide enhances cisplatin sensitivity in non-small cell lung cancer through PUMA-mediated apoptotic pathway

Silencing of LncRNA XIST Suppressed Tumor Growth and Metastasis in Papillary Thyroid Carcinoma by Modulating miR-204/FN1 Axis

Impact of SLC16A8 on tumor microenvironment and angiogenesis in colorectal cancer: New therapeutic target insights

A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

MiR-1290 induces endothelial-to-mesenchymal transition and promotes vascular restenosis after angioplasty by targeting FGF2

Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer

PD-L1 knockdown suppresses vasculogenic mimicry of non-small cell lung cancer by modulating ZEB1-triggered EMT

PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3

SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion

Downregulation of MAL2 inhibits breast cancer progression through regulating β-catenin/c-Myc axis

SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells

STAT5A modulates gastric cancer progression via upregulation of CD44

CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling

Microtubule-associated protein 4 promotes epithelial mesenchymal transition in hepatocellular cancer cells via regulating GSK3β/β-catenin pathway

EMX2OS targeting IGF2BP1 represses Wilms’ tumour stemness, epithelial–mesenchymal transition and metastasis

Discovery of a Novel Bloom’s Syndrome Protein (BLM) Inhibitor Suppressing Growth and Metastasis of Prostate Cancer

DLGAP4 acts as an effective prognostic predictor for hepatocellular carcinoma and is closely related to tumour progression

Lycorine upregulates the expression of RMB10, promotes apoptosis and inhibits the proliferation and migration of cervical cancer cells

Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer

Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter

LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer

Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer

Isoflurane promotes epithelial-to-mesenchymal transition and metastasis of bladder cancer cells through HIF-1α-β-catenin/Notch1 pathways

Immunomodulatory effect of human amniotic epithelial cells on restoration of ovarian function in mice with autoimmune ovarian disease

Hsa_circ_001569 is an unfavorable prognostic factor and promotes cell proliferation and metastasis by modulating PI3K-AKT pathway in breast cancer

Up-regulated circular RNA hsa_circ_0067934 contributes to glioblastoma progression through activating PI3K-AKT pathway

Hsa_circ_0005075 predicts a poor prognosis and acts as an oncogene in colorectal cancer via activating Wnt/β-catenin pathway

MDM2 promotes epithelial–mesenchymal transition through activation of Smad2/3 signaling pathway in lung adenocarcinoma

Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1

Interleukin-27 Inhibits Trophoblast Cell Invasion and Migration by Affecting the Epithelial–Mesenchymal Transition in Preeclampsia

Identification and differentiation therapy strategy of pterygium in vitro

Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells

Anti-cancer effect of Aquaporin 5 silencing in colorectal cancer cells in association with inhibition of Wnt/β-catenin pathway

MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by targeting GAS7

Andrographolide Induces Autophagic Cell Death and Inhibits Invasion and Metastasis of Human Osteosarcoma Cells in An Autophagy-Dependent Manner

Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells

MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial‑mesenchymal transition via the Wnt/β‑catenin pathway

ANXA1 affects cell proliferation, invasion and epithelial‑mesenchymal transition of oral squamous cell carcinoma

Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma

E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin

Different miR-21-3p isoforms and their different features in colorectal cancer

Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

Expression of N-cadherin in myocardial tissues during the development of a rat heart

SOX9 Overexpression Promotes Glioma Metastasis via Wnt/β-Catenin Signaling

Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer

Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/β-catenin pathway in laryngeal cancer Hep-2 cells

Intervention Effects of Ganoderma Lucidum Spores on Epileptiform Discharge Hippocampal Neurons and Expression of Neurotrophin-4 and N-Cadherin

Epithelial-mesenchymal transition in oral squamous cell carcinoma triggered by transforming growth factor-β1 is Snail family-dependent and correlates with matrix metalloproteinase-2 and -9 expressions

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