Product Info Summary
SKU: | A00261-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-NAK/TBK1 Antibody Picoband™
SKU/Catalog Number
A00261-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAK/TBK1 Antibody Picoband™ catalog # A00261-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NAK/TBK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00261-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NAK/TBK1 recombinant protein (Position: M1-L729).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00261-1 is reactive to TBK1 in Human, Mouse, Rat
Applications
A00261-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
84 kDa
Calculated molecular weight
83.642kDa
Background of TBK1
Serine/threonine-protein kinase TBK1, also called TANK-binding kinase 1 or NF-kappa-B-activating kinase is an enzyme that in humans is encoded by the TBK1 gene. The gene was assigned to human chromosome 12q14.2. Serine/threonine kinase plays an essential role in regulating inflammatory responses to foreign agents. TBK1 and NF-kappa-B signaling are essential in KRAS mutant tumors, and established a general approach for the rational identification of codependent pathways in cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (A00261-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human LNCAP whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAK/TBK1 antigen affinity purified polyclonal antibody (Catalog # A00261-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAK/TBK1 at approximately 84 kDa. The expected band size for NAK/TBK1 is at 84 kDa.
Click image to see more details
Figure 2. IHC analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (A00261-1).
NAK/TBK1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAK/TBK1 Antibody (A00261-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TBK1 (Source: Uniprot.org, NCBI)
Gene Name
TBK1
Full Name
Serine/threonine-protein kinase TBK1
Weight
83.642kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; FLJ11330; FTDALS4; NAK; NAK serine; NAK serine/threonine-protein kinase TBK 1; NAK; NAKserine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; NFKB-Activating Kinase; T2K; TANK; TANK binding kinase; TANK-binding kinase 1NF-kB-activating kinase; TBK 1; TBK1 TBK1 FTDALS4, IIAE8, NAK, T2K TANK binding kinase 1 serine/threonine-protein kinase TBK1|NF-kB-activating kinase|NF-kappa-B-activating kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TBK1, check out the TBK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TBK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAK/TBK1 Antibody Picoband™ (A00261-1)
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No publications found for A00261-1
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