Anti-NCOA3/Src3 Antibody

Product Name

Anti-NCOA3/Src3 Antibody

View all NCOA3/AIB1 Antibodies

SKU/Catalog Number

A01337

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NCOA3/Src3 Antibody (Catalog # A01337). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-NCOA3/Src3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01337)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01337 is reactive to NCOA3 in Human

Applications

A01337 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

155.293kDa

Background of NCOA3/AIB1

The NCOA3 (also known as nuclear receptor coactivator 3 isoform a) is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions in a hormone-dependent fashion. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. NCOA3 probably acts via remodeling of chromatin and is involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. NCOA3 may be associated with diseases such as breast cancer and meningothelial meningioma. Anti-NCOA3 Antibody is useful for researchers interested in cancer research, chromatin binding, transcription coactivator activity, Immunology, and Nuclear Signaling research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:175,000
IHC: 5µg/mL
IP: 1:100
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

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NCOA3 was detected in paraffin-embedded sections of human skin spleen tissue using rabbit anti-NCOA3 Antigen Affinity purified polyclonal antibody (Catalog # A01337) at 5 μg/mL. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

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Western blot analysis of NCOA3 expression in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). NCOA3 at 148/105KD was detected using rabbit anti-NCOA3 Antigen Affinity purified polyclonal antibody (Catalog # A01337). The band at 148 kDa corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as 105 kDa bands. Personal communication, H. Ying, NCI, Bethesda, MD.

Gene/Protein Information For NCOA3 (Source: Uniprot.org, NCBI)

Alternative Names

ACTR; ACTRCBP-interacting protein; AIB1; AIB1AIB-1; Amplified in breast cancer 1 protein; BHLHE42; bHLHe42p/CIP; CAGH16; Class E basic helix-loop-helix protein 42; CTG26; KAT13B; MGC141848; NCOA3; NCoA-3; nuclear receptor coactivator 3; pCIP ; pCIP; RAC3; RAC-3; RAC3pCIP; Receptor-associated coactivator 3; SRC-1; SRC3; SRC-3; SRC-3EC 2.3.1.48; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC16; TRAM1; TRAM-1TNRC14 NCOA3 ACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, RAC3, SRC-3, SRC3, TNRC14, TNRC16, TRAM-1, bHLHe42, pCIP nuclear receptor coactivator 3 nuclear receptor coactivator 3|CBP-interacting protein|amplified in breast cancer 1 protein|class E basic helix-loop-helix protein 42|receptor-associated coactivator 3|steroid receptor coactivator protein 3|thyroid hormone receptor activator molecule 1

For more info on NCOA3, check out the NCOA3 Infographic

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In this infographic, you will see the following information for NCOA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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