Product Info Summary
SKU: | A06821 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NEFM Antibody
SKU/Catalog Number
A06821
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NEFM Antibody catalog # A06821. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEFM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06821)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human STK25 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06821 is reactive to NEFM in Human, Mouse, Rat
Applications
A06821 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
102.472kDa
Background of NF-M
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
Kourlas P.J., Proc. Natl. Acad. Sci. U.S.A. 97:2145-2150(2000).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Nagase T., DNA Res. 4:141-150(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of NEFM using Anti-NEFM Antibody (A06821).
ICC staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-NEFM Antibody A06821
Western blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For NEFM (Source: Uniprot.org, NCBI)
Gene Name
NEFM
Full Name
Neurofilament medium polypeptide
Weight
102.472kDa
Superfamily
intermediate filament family
Alternative Names
NEF3; NEF3neurofilament medium polypeptide; NEFM; Neurofilament 3; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide; neurofilament-3 (150 kD medium); NFM; NF-M; NF-M160 kDa neurofilament protein; NFMNeurofilament triplet M protein NEFM NEF3, NF-M, NFM neurofilament medium neurofilament medium polypeptide|160 kDa neurofilament protein|neurofilament 3|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEFM, check out the NEFM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEFM Antibody (A06821)
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No publications found for A06821
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Customer Reviews
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