Anti-Neutral ceramidase ASAH2 Antibody

Product Name

Anti-Neutral ceramidase ASAH2 Antibody

View all ASAH2/N-acylsphingosine Amidohydrolase-2 Antibodies

SKU/Catalog Number

A04725

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Neutral ceramidase ASAH2 Antibody (Catalog # A04725). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ASAH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Neutral ceramidase ASAH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04725)

Host

Rabbit

Contents

ASAH2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2. The immunogen is located within the last 50 amino acids of ASAH2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SLC29A2 antibody is human specific. SLC29A2 antibody is predicted to not cross-react with other SLC29 proteins.

Reactive Species

A04725 is reactive to Asah2 in Human, Mouse, Rat

Applications

A04725 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

83.509kDa

Background of ASAH2/N-acylsphingosine Amidohydrolase-2

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ASAH2 antibody can be used for detection of ASAH2 by Western blot at 1 and 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL.

Gene/Protein Information For Asah2 (Source: Uniprot.org, NCBI)

Alternative Names

ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2; BCDase; HNAC1; LCDase; Nacylsphingosine Amidohydrolase2; NCDase; N-CDase Asah2|AI585898|N-acylsphingosine amidohydrolase 2|neutral ceramidase|N-CDase|NCDase|acylsphingosine deacylase 2|neutral/alkaline ceramidase

For more info on Asah2, check out the Asah2 Infographic

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In this infographic, you will see the following information for Asah2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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