Product Info Summary
SKU: | M00738 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Nkx-2.5 Monoclonal Antibody
SKU/Catalog Number
M00738
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Nkx-2.5 Monoclonal Antibody catalog # M00738. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nkx-2.5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00738)
Host
Mouse
Contents
Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
20
Isotype
IgG
Immunogen
Purified recombinant fragment of human Nkx-2.5 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00738 is reactive to NKX2-5 in Human
Applications
M00738 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34.918kDa
Background of NKX2.5
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using Nkx-2.5 Monoclonal Antibody against full-length NKX2.5 (aa1-324)-hIgGFc transfected HEK293 cell lysate (1).
Protein Target Info & Infographic
Gene/Protein Information For NKX2-5 (Source: Uniprot.org, NCBI)
Gene Name
NKX2-5
Full Name
Homeobox protein Nkx-2.5
Weight
34.918kDa
Superfamily
NK-2 homeobox family
Alternative Names
cardiac-specific homeo box; cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX1; CSXFLJ97195; FLJ52202; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5 (Drosophila); NKX2.5; NKX2.5FLJ97166; NKX2-5; NKX2E; NKX2EFLJ97197; NKX4-1; tinman paralog NKX2-5 CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3 NK2 homeobox 5 homeobox protein Nkx-2.5|NK2 transcription factor related, locus 5|NKX 2-5|cardiac-specific homeobox 1|homeobox protein CSX|homeobox protein NK-2 homolog E|homeobox protein NKX 2-5|tinman homolog|tinman paralog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NKX2-5, check out the NKX2-5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NKX2-5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nkx-2.5 Monoclonal Antibody (M00738)
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No publications found for M00738
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Customer Reviews
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