Click image to see more details
Product Info Summary
| SKU: | M01808 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NMDAR1 GRIN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01808
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NMDAR1 GRIN1 Rabbit Monoclonal Antibody catalog # M01808. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NMDAR1 GRIN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01808)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAHF-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NMDAR1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01808 is reactive to GRIN1 in Human, Mouse, Rat
Applications
M01808 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
105.373kDa
Background of GRIN1
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of NMDAR1 expression in mouse brain lysate.
Protein Target Info & Infographic
Gene/Protein Information For GRIN1 (Source: Uniprot.org, NCBI)
Gene Name
GRIN1
Full Name
Glutamate receptor ionotropic, NMDA 1
Weight
105.373kDa
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
glutamate [NMDA] receptor subunit zeta-1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA1; NMDAR1glutamate [NMDA] receptor subunit zeta 1; NMD-R1; N-methyl-D-aspartate receptor channel, subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1 GRIN1 GluN1, MRD8, NDHMSD, NDHMSR, NMD-R1, NMDA1, NMDAR1, NR1 glutamate ionotropic receptor NMDA type subunit 1 glutamate receptor ionotropic, NMDA 1|N-methyl-D-aspartate receptor channel, subunit zeta-1|N-methyl-D-aspartate receptor subunit NR1|glutamate [NMDA] receptor subunit zeta-1|glutamate receptor, ionotropic, N-methyl D-aspartate 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIN1, check out the GRIN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NMDAR1 GRIN1 Rabbit Monoclonal Antibody (M01808)
Hello CJ!
M01808 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injury-induced coma: orexin-A-mediated N-methyl-D-aspartate receptor subunit NR1 upregulation
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NMDAR1 GRIN1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-NMDAR1 GRIN1 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-NMDAR1 GRIN1 Rabbit Monoclonal Antibody
Question
I would like to know if the epitope binds extracellularly or not.
Verified customer
Asked: 2022-09-12
Answer
The immunogen sequence for M01808 is AITSTLASSFKRR.
Boster Scientific Support
Answered: 2022-09-12
Question
See attached the WB image, lot number and protocol we used for anterior cingulate cortex using anti-NMDAR1 Rabbit Monoclonal antibody M01808. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-NMDAR1 Rabbit Monoclonal antibody M01808. Let me know if you need anything else.
E. Edwards
Verified customer
Asked: 2020-04-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
We have observed staining in human anterior cingulate cortex. Any tips? Is anti-NMDAR1 Rabbit Monoclonal antibody supposed to stain anterior cingulate cortex positively?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
From literature anterior cingulate cortex does express GRIN1. From Uniprot.org, GRIN1 is expressed in anterior cingulate cortex, brain, cerebellum hippocampus, among other tissues. Regarding which tissues have GRIN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7685113, 8406025
Cerebellum, and Hippocampus, Pubmed ID: 7926821
Boster Scientific Support
Answered: 2020-02-12
Question
Would anti-NMDAR1 Rabbit Monoclonal antibody M01808 work for WB with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
According to the expression profile of anterior cingulate cortex, GRIN1 is highly expressed in anterior cingulate cortex. So, it is likely that anti-NMDAR1 Rabbit Monoclonal antibody M01808 will work for WB with anterior cingulate cortex.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-NMDAR1 Rabbit Monoclonal antibody M01808 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The anti-NMDAR1 Rabbit Monoclonal antibody (M01808) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-12
Question
I was wanting to use your anti-NMDAR1 Rabbit Monoclonal antibody for WB for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse anterior cingulate cortex identification?
L. Li
Verified customer
Asked: 2019-11-29
Answer
As indicated on the product datasheet, M01808 anti-NMDAR1 Rabbit Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-29
Question
Can you help my question with product M01808, anti-NMDAR1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Moore
Verified customer
Asked: 2019-10-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01808 anti-NMDAR1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-07
Question
We are interested in using your anti-NMDAR1 Rabbit Monoclonal antibody for ephb-mediated forward signaling studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We appreciate your inquiry. This M01808 anti-NMDAR1 Rabbit Monoclonal antibody is validated on mouse brain. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-30
Question
My boss were satisfied with the WB result of your anti-NMDAR1 Rabbit Monoclonal antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is GRIN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
From literature, brain does express GRIN1. Generally GRIN1 expresses in cell membrane. Regarding which tissues have GRIN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7685113, 8406025
Cerebellum, and Hippocampus, Pubmed ID: 7926821
Boster Scientific Support
Answered: 2019-08-02
Question
I see that the anti-NMDAR1 Rabbit Monoclonal antibody M01808 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-04
Question
Is there a BSA free version of anti-NMDAR1 Rabbit Monoclonal antibody M01808 available?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NMDAR1 Rabbit Monoclonal antibody M01808 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-12
Question
Is this M01808 anti-NMDAR1 Rabbit Monoclonal antibody reactive to the isotypes of GRIN1?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
The immunogen of M01808 anti-NMDAR1 Rabbit Monoclonal antibody is A synthesized peptide derived from human NMDAR1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-24
Question
Does M01808 anti-NMDAR1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Bhatt
Verified customer
Asked: 2015-03-24
Answer
As indicated on the product datasheet, M01808 anti-NMDAR1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-24
Question
Is a blocking peptide available for product anti-NMDAR1 Rabbit Monoclonal antibody (M01808)?
N. Zhang
Verified customer
Asked: 2014-04-28
Answer
We do provide the blocking peptide for product anti-NMDAR1 Rabbit Monoclonal antibody (M01808). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-04-28
Question
We are interested in to test anti-NMDAR1 Rabbit Monoclonal antibody M01808 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-NMDAR1 Rabbit Monoclonal antibody M01808 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Anderson
Verified customer
Asked: 2013-03-08
Answer
The products we sell, including anti-NMDAR1 Rabbit Monoclonal antibody M01808, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-08
