Product Info Summary
SKU: | PA1058-1 |
---|---|
Size: | 100μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-NMDAR2A/GRIN2A Antibody
SKU/Catalog Number
PA1058-1
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NMDAR2A/GRIN2A Antibody catalog # PA1058-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NMDAR2A/GRIN2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1058-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NMDAR2A, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
PA1058-1 is reactive to GRIN2A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
165283 MW
Background of GRIN2A
GRIN2A is also known as N-methyl-D-aspartate receptor channel, subunit epsilon-1 (NMDAR2A). This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1058-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-NMDAR2A antibody, PA1058-1, Western blotting
All lanes: Anti NMDAR2A (PA1058-1) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 165KD
Observed bind size: 165KD
Click image to see more details
Anti-NMDAR2A antibody, PA1058-1, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For GRIN2A (Source: Uniprot.org, NCBI)
Gene Name
GRIN2A
Full Name
Glutamate receptor ionotropic, NMDA 2A
Weight
165283 MW
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
Glutamate receptor ionotropic, NMDA 2A;GluN2A;Glutamate [NMDA] receptor subunit epsilon-1;N-methyl D-aspartate receptor subtype 2A;NMDAR2A;NR2A;hNR2A;GRIN2A;NMDAR2A; GRIN2A EPND, FESD, GluN2A, LKS, NMDAR2A, NR2A glutamate ionotropic receptor NMDA type subunit 2A glutamate receptor ionotropic, NMDA 2A|N-methyl D-aspartate receptor subtype 2A|N-methyl-D-aspartate receptor channel, subunit epsilon-1|N-methyl-D-aspartate receptor subunit 2A|glutamate ionotropic receptor NMDA 2A|glutamate receptor, ionotropic, N-methyl D-aspartate 2A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIN2A, check out the GRIN2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIN2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NMDAR2A/GRIN2A Antibody (PA1058-1)
Hello CJ!
PA1058-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Impaired hippocampal synaptic plasticity and NR2A/2B expression ratio in remifentanil withdrawal rats
Liang Y,Ma Y,Wang J,Nie L,Hou X,Wu W,Zhang X,Tian Y. Leptin Contributes to Neuropathic Pain via Extrasynaptic NMDAR-nNOS Activation. Mol Neurobiol.2020 Oct 25.doi:10.1007/s12035-020-02180-1.Epub ahead of print.PMID:33099751.
Species: Rat
PA1058-1 usage in article: APP:WB, SAMPLE:DRG NEURONS, DILUTION:1:400
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Customer Q&As
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6 Customer Q&As for Anti-NMDAR2A/GRIN2A Antibody
Question
Would anti-NMDAR2A/GRIN2A antibody PA1058-1 work for IHC with cerebellum?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
According to the expression profile of cerebellum, GRIN2A is highly expressed in cerebellum. So, it is likely that anti-NMDAR2A/GRIN2A antibody PA1058-1 will work for IHC with cerebellum.
Boster Scientific Support
Answered: 2019-12-18
Question
Our lab want to know about to test anti-NMDAR2A/GRIN2A antibody PA1058-1 on mouse cerebellum for research purposes, then I may be interested in using anti-NMDAR2A/GRIN2A antibody PA1058-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The products we sell, including anti-NMDAR2A/GRIN2A antibody PA1058-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-02
Question
Is this PA1058-1 anti-NMDAR2A/GRIN2A antibody reactive to the isotypes of GRIN2A?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
The immunogen of PA1058-1 anti-NMDAR2A/GRIN2A antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human NMDAR2A(1360-1376aa, DHTSDNPFLHSHRDDQR), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-27
Question
Do you have a BSA free version of anti-NMDAR2A/GRIN2A antibody PA1058-1 available?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-NMDAR2A/GRIN2A antibody PA1058-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-15
Question
I see that the anti-NMDAR2A/GRIN2A antibody PA1058-1 works with IHC, what is the protocol used to produce the result images on the product page?
P. Baker
Verified customer
Asked: 2015-06-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-06-01
Question
I was wanting to use your anti-NMDAR2A/GRIN2A antibody for IHC for mouse cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cerebellum identification?
D. Roberts
Verified customer
Asked: 2015-02-24
Answer
It shows on the product datasheet, PA1058-1 anti-NMDAR2A/GRIN2A antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-24