Anti-NOTCH 2 Antibody

Product Name

Anti-NOTCH 2 Antibody

View all Notch-2 Antibodies

SKU/Catalog Number

A00518

Size

200uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NOTCH 2 Antibody (Catalog # A00518). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-NOTCH 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00518)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.1% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa).  A residue of cysteine was added to the amino terminal end to facilitate coupling.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00518 is reactive to NOTCH2 in Human

Applications

A00518 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

265.405kDa

Background of Notch-2

Anti Notch 2 Antibody recognizes Notch 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:30,000 - 1:90,000
IHC: 1:200-1:800
WB: 1:400 - 1:2,000

Validation Images & Assay Conditions

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Anti-Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue.Tissue was formalin fixed and paraffin embedded.No pre-treatment of sample was required.The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

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Figure 2. Western blot analysis of NOTCH2 using anti-NOTCH2 antibody (A00518).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH2 antigen affinity purified polyclonal antibody (Catalog # A00518) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.

Gene/Protein Information For NOTCH2 (Source: Uniprot.org, NCBI)

Alternative Names

AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2 NOTCH2 AGS2, HJCYS, hN2 notch receptor 2 neurogenic locus notch homolog protein 2|Notch homolog 2|notch 2

For more info on NOTCH2, check out the NOTCH2 Infographic

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In this infographic, you will see the following information for NOTCH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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