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Product Info Summary
| SKU: | A00428 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NPC1 Antibody
View all Niemann-Pick C1 Antibodies
SKU/Catalog Number
A00428
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NPC1 Antibody (Catalog # A00428). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
NPC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NPC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00428)
Host
Rabbit
Contents
NPC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
NPC1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human NPC1. The immunogen is located within the last 50 amino acids of NPC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ALSFTD antibody is human, mouse and rat reactive. At least two isoforms are known to exist.
Reactive Species
A00428 is reactive to NPC1 in Human, Mouse
Applications
A00428 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
142.167kDa
Background of Niemann-Pick C1
Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations. This anti-NPC1 antibody will not cross-react to NPC2, another gene whose defects also result in Niemann-Pick type C disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NPC1 antibody can be used for detection of NPC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NPC1 in HepG2 cell lysate with NPC1 antibody at 1 μg/mL.
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Immunohistochemistry of NPC1 in mouse kidney tissue with NPC1 antibody at 2.5 μg/mL.
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Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NPC1 (Source: Uniprot.org, NCBI)
Gene Name
NPC1
Full Name
NPC intracellular cholesterol transporter 1
Weight
142.167kDa
Superfamily
patched family
Alternative Names
FLJ98532; Niemann-Pick C1 protein; Niemann-Pick disease, type C1; Niemann-Pick Type C1; NPC; NPC1; SLC65A1 NPC1 NPC, POGZ, SLC65A1 NPC intracellular cholesterol transporter 1 NPC intracellular cholesterol transporter 1|Niemann-Pick C1 protein|truncated Niemann-Pick C1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPC1, check out the NPC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NPC1 Antibody (A00428)
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Customer Reviews
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