Anti-NPDC-1 Antibody

NPDC-1 antibody

Boster Bio Anti-NPDC-1 Antibody catalog # A12846-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A12846-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-NPDC-1 Antibody

View all NPDC-1 Antibodies

SKU/Catalog Number

A12846-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-NPDC-1 Antibody catalog # A12846-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NPDC-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12846-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2H4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human NPDC-1. at AA rangle: 160-240

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12846-1 is reactive to Npdc1 in Human, Mouse, Rat

Applications

A12846-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

35.72kDa

Background of NPDC-1

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Npdc1 (Source: Uniprot.org, NCBI)

Gene Name

Npdc1

Full Name

Neural proliferation differentiation and control protein 1

Weight

35.72kDa

Superfamily

NPDC1/cab-1 family

Alternative Names

CAB-; CAB1; CAB-1; CAB1CAB; DKFZp586J0523; neural proliferation differentiation and control protein 1; neural proliferation, differentiation and control, 1; NPDC1; NPDC-1 Npdc1|AI314472, NPDC, NPDC-1|neural proliferation, differentiation and control 1|neural proliferation differentiation and control protein 1|neural proliferation differentiation control-1 protein|neural proliferation, differentiation and control gene 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Npdc1, check out the Npdc1 Infographic

Npdc1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Npdc1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-NPDC-1 Antibody

Question

I see that the anti-NPDC-1 antibody A12846-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-09

Question

We are currently using anti-NPDC-1 antibody A12846-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

The anti-NPDC-1 antibody (A12846-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-31

Question

See attached the WB image, lot number and protocol we used for uterus using anti-NPDC-1 antibody A12846-1. Please let me know if you require anything else.

G. Edwards

Verified customer

Asked: 2018-09-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-03

Question

I was wanting to use your anti-NPDC-1 antibody for WB for mouse uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse uterus identification?

D. Johnson

Verified customer

Asked: 2017-11-03

Answer

It shows on the product datasheet, A12846-1 anti-NPDC-1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-03

Question

Will anti-NPDC-1 antibody A12846-1 work on canine WB with iris?

B. Krishna

Verified customer

Asked: 2013-03-18

Answer

Our lab technicians have not validated anti-NPDC-1 antibody A12846-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-NPDC-1 antibody A12846-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine iris in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-03-18

Order DetailsPrice
A12846-1

100uL

$399

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