Click image to see more details
Product Info Summary
| SKU: | PA1322 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Podocin NPHS2 Antibody
View all Podocin/NPHS2 Antibodies
SKU/Catalog Number
PA1322
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Podocin NPHS2 Antibody catalog # PA1322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Podocin NPHS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1322)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NPHS2, different from the mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1322 is reactive to NPHS2 in Human, Mouse, Rat
Applications
PA1322 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
42.201kDa
Background of Podocin/NPHS2
Podocin (PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al. (2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-NPHS2 antibody, PA1322, Western blotting
WB: Rat Kidney Tissue Lysate
Protein Target Info & Infographic
Gene/Protein Information For NPHS2 (Source: Uniprot.org, NCBI)
Gene Name
NPHS2
Full Name
Podocin
Weight
42.201kDa
Superfamily
band 7/mec-2 family
Alternative Names
Nephrosis 2; nephrosis 2, idiopathic, steroid-resistant (podocin); NPHS2; PDCN; PDCNSRN1podocin; Podocin; SRN1 NPHS2 PDCN, SRN1 NPHS2 stomatin family member, podocin podocin|NPHS2, podocin|nephrosis 2, idiopathic, steroid-resistant (podocin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPHS2, check out the NPHS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPHS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Podocin NPHS2 Antibody (PA1322)
Hello CJ!
PA1322 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Qiu Y,Zhou J,Zhang H,Zhou H,Tang H,Lei C,Ye C,You C,Chen Y,Wang Y,Xiong J,Su H,Yao G,Zhang C.Rhodojaponin II attenuates kidney injury by regulating TGF-β1/Smad pathway in mice with adriamycin nephropathy.J Ethnopharmacol.2019 Oct 28;243:112078.doi:10.1016/j.jep.2019.112078.Epub 2019 Jul 10.PMID: 31301369.
Species: Mouse
PA1322 usage in article: APP:WB, SAMPLE:RENAL TISSUE, DILUTION:1:200
Zhang X,Zhang L,Chen Z,Li S,Che B, Wang N,Chen J,Xu C,Wei C.Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.Int J Mol Med.2021 Mar;47(3):27.doi:10.3892/ijmm.2021.4860.Epub 2021 Feb 4.PMID:33537831.
Species: Rat
PA1322 usage in article: APP:WB, SAMPLE:RENAL TISSUE AND PODOCYTES, DILUTION:1:400
Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
PA1322 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000
Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
Effect of simvastatin on the expression of nephrin, podocin, and vascular endothelial growth factor (VEGF) in podocytes of diabetic rat
Hao L, Pan Ms, Zheng Y, Wang Rf. Exp Ther Med. 2014 Jun;7(6):1465-1470. Epub 2014 Apr 7. Effect Of Cordyceps Sinensis And Tripterygium Wilfordii Polyglycosidium On Podocytes In Rats With Diabetic Nephropathy.
Qu Y, Du E, Zhang Y, Li S, Han R, Qiu M. Nephrourol Mon. 2012 Spring;4(2):466-9. Doi: 10.5812/Numonthly.2124. Epub 2012 Mar 1. Changes In The Expression Of Bone Morphogenetic Protein 7 And Tamm- Horsfall Protein In The Early Stages Of Diabetic Nep...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Anti-Podocin NPHS2 Antibody
Question
Is a blocking peptide available for product anti-NPHS2 antibody (PA1322)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We do provide the blocking peptide for product anti-NPHS2 antibody (PA1322). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-01
Question
I see that the anti-NPHS2 antibody PA1322 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-13
Question
I was wanting to use your anti-NPHS2 antibody for WB for human renal glomerulus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human renal glomerulus identification?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
It shows on the product datasheet, PA1322 anti-NPHS2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human renal glomerulus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for renal glomerulus using anti-NPHS2 antibody PA1322. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
Is this PA1322 anti-NPHS2 antibody reactive to the isotypes of NPHS2?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The immunogen of PA1322 anti-NPHS2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human NPHS2(126-143aa CVKVVQEYERVIIFRLGH), different from the mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-17
Question
Will PA1322 anti-NPHS2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
You can see on the product datasheet, PA1322 anti-NPHS2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-16
Question
I have a question about product PA1322, anti-NPHS2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1322 anti-NPHS2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-15
