Anti-OIP5 Antibody Picoband™

Product Name

Anti-OIP5 Antibody Picoband™

View all OIP5 Antibodies

SKU/Catalog Number

A07819

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-OIP5 Antibody Picoband™ catalog # A07819. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-OIP5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07819)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human OIP5 recombinant protein (Position: M1-N229).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07819 is reactive to OIP5 in Human

Applications

A07819 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

24.691kDa

Background of OIP5

The protein encoded by this gene localizes to centromeres, where it is essential for recruitment of CENP-A through the mediator Holliday junction recognition protein. Expression of this gene is upregulated in several cancers, making it a putative therapeutic target. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of OIP5 using anti-OIP5 antibody (A07819).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIP5 antigen affinity purified polyclonal antibody (Catalog # A07819) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIP5 at approximately 22 kDa. The expected band size for OIP5 is at 25 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-OIP5 antibody (A07819).
Overlay histogram showing HepG2 cells stained with A07819 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OIP5 Antibody (A07819, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For OIP5 (Source: Uniprot.org, NCBI)

Alternative Names

Cancer/testis antigen 86; CT865730547N13Rik; hMIS18beta; LAP2alpha interactor-25; LINT-25; MIS18B; MIS18beta; OIP-5; Opa interacting protein 5; Opa-interacting protein 5; protein Mis18-beta OIP5 5730547N13Rik, CT86, LINT-25, MIS18B, MIS18beta, hMIS18beta Opa interacting protein 5 protein Mis18-beta|LAP2alpha interactor-25|MIS18 kinetochore protein homolog B|cancer/testis antigen 86|opa-interacting protein 5

For more info on OIP5, check out the OIP5 Infographic

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In this infographic, you will see the following information for OIP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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