Click image to see more details
Product Info Summary
| SKU: | A00016-3 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-p16 CDKN2A Monoclonal Antibody
SKU/Catalog Number
A00016-3
Size
100μl
Form
Liquid
Description
Boster Bio Anti-p16 CDKN2A Monoclonal Antibody catalog # A00016-3. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p16 CDKN2A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00016-3)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Clonality
Monoclonal
Clone Number
1E12E1
Isotype
IgG1
Immunogen
Purified recombinant fragment of p16 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00016-3 is reactive to CDKN2A in Human, Mouse, Rat
Applications
A00016-3 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
16.533kDa
Background of CDKN2A
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene (isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA 1:10000
IHC 1:200-1:1000
WB 1:500-1:2000
Validation Images & Assay Conditions
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Western blot analysis using p16 monoclonal antibody against truncated p16 recombinant protein.
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Immunohistochemistry analysis of paraffin-embedded rat liver tissue (A) , human brain tumor (B) , breast cancer (C) , esophageal epithelium tissue (D) , mouse brain tissue (E) and stomach tisue (F) , showing nuclear localization with DAB staining using p16 Mon
Protein Target Info & Infographic
Gene/Protein Information For CDKN2A (Source: Uniprot.org, NCBI)
Gene Name
CDKN2A
Full Name
Cyclin-dependent kinase inhibitor 2A
Weight
16.533kDa
Superfamily
CDKN2 cyclin-dependent kinase inhibitor family
Alternative Names
alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16 cyclin dependent kinase inhibitor 2A cyclin-dependent kinase inhibitor 2A|CDK4 inhibitor p16-INK4|alternative reading frame|cell cycle negative regulator beta|cyclin-dependent kinase 4 inhibitor A|cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)|multiple tumor suppressor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN2A, check out the CDKN2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p16 CDKN2A Monoclonal Antibody (A00016-3)
Hello CJ!
A00016-3 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
P53 and Parkin co-regulate mitophagy in bone marrow mesenchymal stem cells to promote the repair of early steroid-induced osteonecrosis of the femoral head
Expression of p16 protein in infantile hemangioma
Song Lj, Liu Rj, Zeng Z, Alper Sl, Cui Hj, Lu Y, Zheng L, Yan Zw, Fu Gh. J Mol Med (Berl). 2012 Jun;90(6):707-18. Doi: 10.1007/S00109-011-0851-2. Epub 2012 Jan 7. Gastrin Inhibits A Novel, Pathological Colon Cancer Signaling Pathway Involving Egr1...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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12 Customer Q&As for Anti-p16 CDKN2A Monoclonal Antibody
Question
My question regards to test anti-p16 Monoclonal antibody A00016-3 on rat adenohypophysis for research purposes, then I may be interested in using anti-p16 Monoclonal antibody A00016-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The products we sell, including anti-p16 Monoclonal antibody A00016-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-08
Question
Is there a BSA free version of anti-p16 Monoclonal antibody A00016-3 available?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p16 Monoclonal antibody A00016-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-09
Question
Does A00016-3 anti-p16 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
As indicated on the product datasheet, A00016-3 anti-p16 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-02
Question
Will anti-p16 Monoclonal antibody A00016-3 work for IHC with adenohypophysis?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16 Monoclonal antibody A00016-3 will work for IHC with adenohypophysis.
Boster Scientific Support
Answered: 2020-01-03
Question
I see that the anti-p16 Monoclonal antibody A00016-3 works with IHC, what is the protocol used to produce the result images on the product page?
M. Mangal
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-09
Question
Is this A00016-3 anti-p16 Monoclonal antibody reactive to the isotypes of CDKN2A?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The immunogen of A00016-3 anti-p16 Monoclonal antibody is Purified recombinant fragment of p16 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-p16 Monoclonal antibody (A00016-3)?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We do provide the blocking peptide for product anti-p16 Monoclonal antibody (A00016-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-24
Question
My question regarding product A00016-3, anti-p16 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00016-3 anti-p16 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-28
Question
See below the WB image, lot number and protocol we used for adenohypophysis using anti-p16 Monoclonal antibody A00016-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-14
Question
I was wanting to use your anti-p16 Monoclonal antibody for IHC for rat adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adenohypophysis identification?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
You can see on the product datasheet, A00016-3 anti-p16 Monoclonal antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-26
Question
We are currently using anti-p16 Monoclonal antibody A00016-3 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
T. Johnson
Verified customer
Asked: 2018-02-09
Answer
The anti-p16 Monoclonal antibody (A00016-3) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16 Monoclonal antibody A00016-3. Let me know if you need anything else.
D. Taylor
Verified customer
Asked: 2013-11-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-22
