Anti-p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Monoclonal Antibody

Product Name

Anti-p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Monoclonal Antibody

View all p57 Kip2 Antibodies

SKU/Catalog Number

M01244

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Monoclonal Antibody (Catalog # M01244). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01244)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 57P06

Isotype

IgG2b, kappa

Immunogen

Recombinant full-length human p57Kip2 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01244 is reactive to CDKN1C in Human, Mouse

Applications

M01244 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

32.177kDa

Background of p57 Kip2

Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Anti-p57 Mouse Monoclonal Antibody (57P06).

Gene/Protein Information For CDKN1C (Source: Uniprot.org, NCBI)

Alternative Names

Beckwith-Wiedemann syndrome; BWS; cyclin-dependent kinase inhibitor 1C (p57, Kip2); cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; KIP2BWCR; p57; p57Kip2; WBS CDKN1C BWCR, BWS, KIP2, WBS, p57, p57Kip2 cyclin dependent kinase inhibitor 1C cyclin-dependent kinase inhibitor 1C|cyclin-dependent kinase inhibitor 1C (p57, Kip2)|cyclin-dependent kinase inhibitor p57

For more info on CDKN1C, check out the CDKN1C Infographic

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In this infographic, you will see the following information for CDKN1C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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