Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26)

KRT8 antibody

Boster Bio Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) catalog # MA1082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: MA1082
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Mouse
Application: IF, IHC, WB

Product Name

Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26)

View all KRT8 Antibodies

SKU/Catalog Number

MA1082

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) catalog # MA1082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

PCK-26

Isotype

Mouse IgG1

Immunogen

Cytokeratin from human epidermis.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1082 is reactive to KRT8 in Human, Mouse

Applications

MA1082 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

53.704kDa

Background of KRT8

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, mouse
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunofluorescence, 1-2μg/ml, Human, -

Validation Images & Assay Conditions

Gene/Protein Information For KRT8 (Source: Uniprot.org, NCBI)

Gene Name

KRT8

Full Name

Keratin, type II cytoskeletal 8

Weight

53.704kDa

Superfamily

intermediate filament family

Alternative Names

CARD2; CK8; CK-8; CYK8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8 KRT8 CARD2, CK-8, CK8, CYK8, K2C8, K8, KO keratin 8 keratin, type II cytoskeletal 8|cytokeratin-8|keratin 8, type II|type-II keratin Kb8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KRT8, check out the KRT8 Infographic

KRT8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KRT8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26)

Question

Does anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 work on zebrafish WB with testis?

Verified Customer

Verified customer

Asked: 2019-04-01

Answer

Our lab technicians have not tested anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-01

Question

See below the WB image, lot number and protocol we used for colon using anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082. Please let me know if you require anything else.

P. Williams

Verified customer

Asked: 2015-12-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-29

Question

I see that the anti-PCK-26 antibody (Monoclonal, PCK-26) MA1082 works with WB, what is the protocol used to produce the result images on the product page?

J. Miller

Verified customer

Asked: 2013-05-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-05-20

Total: $370

SKU:MA1082

Backordered.

Lead time for this item is typically 2-3 weeks

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MA1082
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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