Anti-PC2 CBX4 Antibody

Product Name

Anti-PC2 CBX4 Antibody

View all CBX4 Antibodies

SKU/Catalog Number

A03388-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PC2 CBX4 Antibody (Catalog # A03388-1). Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-PC2 CBX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03388-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03388-1 is reactive to CBX4 in Human

Applications

A03388-1 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

61.368kDa

Background of CBX4

PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to depression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:40,000
IF Microscopy: 1:100 - 1:400
IP: 1:100
WB: 1:1,000 - 1:4,000

Validation Images & Assay Conditions

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Analysis shows the detection of human PC2 in probed lysates using Affinity Purified anti-hPC2 antibody.The panel on the left shows the blot probed with anti-hPC2.The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant PC2 in the lysate. In the left panel the band labeled as hPC2 is FLAG-tagged transfected hPC2 in 293T cells. The bands labeled with stars are likely endogenous hPC2.In the right panel the band labeled hPC2 is FLAG-tagged transfected hPC2 in 293T cells. Data contributed by Dr Ari Melnick, Albert Einstein College of Medicine.

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Anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS).The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2 (see Satijn et al. below).IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Triton X100-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37

Gene/Protein Information For CBX4 (Source: Uniprot.org, NCBI)

Alternative Names

chromobox homolog 4 (Drosophila Pc class); chromobox homolog 4; Chromobox protein homolog 4; Drosophila); E3 SUMO-protein ligase CBX4; hPC2; NS5ATP1-binding protein 16; Pc class 2 homolog; PC2; Polycomb 2 homolog CBX4 NBP16, PC2 chromobox 4 E3 SUMO-protein ligase CBX4|NS5ATP1-binding protein 16|Pc class 2 homolog|chromobox homolog 4 (Pc class homolog, Drosophila)|chromobox protein homolog 4|chromobox-like protein 4|polycomb 2 homolog

For more info on CBX4, check out the CBX4 Infographic

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In this infographic, you will see the following information for CBX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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