Click image to see more details
Product Info Summary
| SKU: | A02403 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PCAF KAT2B Antibody
SKU/Catalog Number
A02403
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PCAF KAT2B Antibody catalog # A02403. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCAF KAT2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02403)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p300/CBP. AA range:783-832
Reactive Species
A02403 is reactive to KAT2B in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
93.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02403 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using p300/CBP Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using PCAF Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from COLO205 cells, using p300/CBP Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PCAF KAT2B Antibody (A02403)
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Customer Q&As
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7 Customer Q&As for Anti-PCAF KAT2B Antibody
Question
Would anti-PCAF antibody A02403 work on horse WB with corpus callosum?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Our lab technicians have not validated anti-PCAF antibody A02403 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PCAF antibody A02403 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse corpus callosum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
Does A02403 anti-PCAF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Gonzalez
Verified customer
Asked: 2018-10-26
Answer
It shows on the product datasheet, A02403 anti-PCAF antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-26
Question
My question regarding product A02403, anti-PCAF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02403 anti-PCAF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PCAF antibody A02403. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-12
Question
My lab would like to test anti-PCAF antibody A02403 on rat liver for research purposes, then I may be interested in using anti-PCAF antibody A02403 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The products we sell, including anti-PCAF antibody A02403, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-02
Question
I see that the anti-PCAF antibody A02403 works with WB, what is the protocol used to produce the result images on the product page?
W. Taylor
Verified customer
Asked: 2014-01-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-01-24
Question
Is there a BSA free version of anti-PCAF antibody A02403 available?
L. Patel
Verified customer
Asked: 2013-02-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PCAF antibody A02403 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-22
