Anti-PCNA Antibody (Monoclonal, PC 10)

Product Name

Anti-PCNA Antibody (Monoclonal, PC 10)

View all PCNA Antibodies

SKU/Catalog Number

MA1083

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCNA Antibody (Monoclonal, PC 10) catalog # MA1083. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PCNA Antibody (Monoclonal, PC 10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1083)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

PC 10

Isotype

Mouse IgG2a

Immunogen

Protein A fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1083 is reactive to PCNA in Human, Mouse, Rat

Applications

MA1083 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

28.769kDa

Background of PCNA

Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (MA1083).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human MDA-MB-231 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HT1080 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PCNA antigen affinity purified monoclonal antibody (Catalog # MA1083) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCNA at approximately 35KD. The expected band size for PCNA is at 29KD.

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Figure 2. IHC analysis of PCNA using anti-PCNA antibody (MA1083).
PCNA was detected in paraffin-embedded section of human Rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Figure 3. IHC analysis of PCNA using anti-PCNA antibody (MA1083).
PCNA was detected in immunocytochemical section of human HELA Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For PCNA (Source: Uniprot.org, NCBI)

Alternative Names

cyclin; DNA polymerase delta auxiliary protein; MGC8367; proliferating cell nuclear antigen PCNA ATLD2 proliferating cell nuclear antigen proliferating cell nuclear antigen|DNA polymerase delta auxiliary protein|cyclin

For more info on PCNA, check out the PCNA Infographic

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In this infographic, you will see the following information for PCNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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