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Product Info Summary
| SKU: | A00178-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-PD-1 PDCD1 Antibody
SKU/Catalog Number
A00178-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PD-1 PDCD1 Antibody (Catalog # A00178-1). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PD-1 PDCD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00178-1)
Host
Rabbit
Contents
PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1. The immunogen is located within amino acids 120 - 170 of PD-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of IRF3 are known to exist.
Reactive Species
A00178-1 is reactive to PDCD1 in Human, Mouse, Rat
Applications
A00178-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.647kDa
Background of PD-1
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
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Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 μg/mL.
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Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 μg/ml.
Green: PD-1 Antibody (A00178-1)
Blue: DAPI staining
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Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PDCD1 (Source: Uniprot.org, NCBI)
Gene Name
PDCD1
Full Name
Programmed cell death protein 1
Weight
31.647kDa
Alternative Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2 PDCD1 CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1 programmed cell death 1 programmed cell death protein 1|programmed cell death 1 protein|protein PD-1|systemic lupus erythematosus susceptibility 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD1, check out the PDCD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PD-1 PDCD1 Antibody (A00178-1)
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No publications found for A00178-1
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