Anti-PDE6 beta/PDE6B Antibody Picoband™ (monoclonal, 8I2D7)

Product Name

Anti-PDE6 beta/PDE6B Antibody Picoband™ (monoclonal, 8I2D7)

View all PDE6 beta Antibodies

SKU/Catalog Number

M02659-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PDE6 beta/PDE6B Antibody Picoband™ (monoclonal, 8I2D7) catalog # M02659-1. Tested in FCM, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PDE6 beta/PDE6B Antibody Picoband™ (monoclonal, 8I2D7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02659-1)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

8I2D7

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human PDE6 beta/PDE6B recombinant protein (Position: K25-Q237).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02659-1 is reactive to PDE6B in Human, Mouse, Rat

Applications

M02659-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

98 kDa

Calculated molecular weight

98.336kDa

Background of PDE6 beta

Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PDE6 beta/PDE6B using anti-PDE6 beta/PDE6B antibody (M02659-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat eye tissue lysates,
Lane 2: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDE6 beta/PDE6B antigen affinity purified monoclonal antibody (Catalog # M02659-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDE6 beta/PDE6B at approximately 98 kDa. The expected band size for PDE6 beta/PDE6B is at 98 kDa.

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Figure 2. IHC analysis of PDE6 beta/PDE6B using anti-PDE6 beta/PDE6B antibody (M02659-1).
PDE6 beta/PDE6B was detected in a paraffin-embedded section of rat eye ball tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDE6 beta/PDE6B Antibody (M02659-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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Figure 3. IF analysis of PDE6 beta/PDE6B using anti-PDE6 beta/PDE6B antibody (M02659-1).
PDE6 beta/PDE6B was detected in an immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-PDE6 beta/PDE6B Antibody (M02659-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 4. Flow Cytometry analysis of U20S cells using anti-PDE6 beta/PDE6B antibody (M02659-1).
Overlay histogram showing U20S cells stained with M02659-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PDE6 beta/PDE6B Antibody (M02659-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PDE6B (Source: Uniprot.org, NCBI)

Alternative Names

CSNB3; EC 3.1.4; EC 3.1.4.35; GMP-PDE beta; PDEBrod cGMP-phosphodiesterase beta-subunit; phosphodiesterase 6B, cGMP-specific, rod, beta; rd1; rod cGMP-specific 3'-5'-cyclic phosphodiesterase subunit beta; RP40CSNBAD2 PDE6B CSNB3, CSNBAD2, GMP-PDEbeta, PDEB, RP40, rd1 phosphodiesterase 6B rod cGMP-specific 3,5-cyclic phosphodiesterase subunit beta|GMP-PDE beta|phosphodiesterase 6B, cGMP-specific, rod, beta|rod cGMP-phosphodiesterase beta-subunit

For more info on PDE6B, check out the PDE6B Infographic

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In this infographic, you will see the following information for PDE6B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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