Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated

Protein Disulfide Isomerase/P4HB antibody

Boster Bio Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated (Catalog# E02335). Tested in Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus.

Product Info Summary

SKU: E02335
Size: 200μg/vial
Reactive Species: Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Porcine
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated

View all Protein Disulfide Isomerase/P4HB Antibodies

SKU/Catalog Number

E02335

Size

200μg/vial

Form

Liquid

Description

Boster Bio Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated (Catalog# E02335). Tested in Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # E02335)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

1D3

Isotype

IgG1

Immunogen

Synthetic peptide corresponding to the sequence near the C-terminus of rat PDI

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

E02335 is reactive to P4HB in Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Porcine

Applications

E02335 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

57.116kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1:100)
Detects a band of ~58kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Gene/Protein Information For P4HB (Source: Uniprot.org, NCBI)

Gene Name

P4HB

Full Name

Protein disulfide-isomerase

Weight

57.116kDa

Superfamily

protein disulfide isomerase family

Alternative Names

Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; DSI; ERBA2L; GIT; glutathione-insulin transhydrogenase; P4HB; P4Hbeta; p55; PDI; PDIA1; PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1); PDIEC 5.3.4.1; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide; PROHB; PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p5 P4HB CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB prolyl 4-hydroxylase subunit beta protein disulfide-isomerase|cellular thyroid hormone-binding protein|collagen prolyl 4-hydroxylase beta|glutathione-insulin transhydrogenase|p55|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide|prolyl 4-hydroxylase, beta polypeptide|protein disulfide isomerase family A, member 1|protein disulfide isomerase-associated 1|protein disulfide isomerase/oxidoreductase|protocollagen hydroxylase|testicular secretory protein Li 32|thyroid hormone-binding protein p55

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on P4HB, check out the P4HB Infographic

P4HB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for P4HB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung skin using anti-PDI antibody (Monoclonal, 1D3), PE Conjugated E02335. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-30

Question

We are currently using anti-PDI antibody (Monoclonal, 1D3), PE Conjugated E02335 for mouse tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

The anti-PDI antibody (Monoclonal, 1D3), PE Conjugated (E02335) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-26

Question

My question regarding product E02335, anti-PDI antibody (Monoclonal, 1D3), PE Conjugated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free E02335 anti-PDI antibody (Monoclonal, 1D3), PE Conjugated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-06

Order DetailsPrice
E02335

200ug

$847

Free Secondary Antibody

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E02335
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$847.00

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