Product Info Summary
SKU: | M00109-3 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-PDL1 CD274 Monoclonal Antibody [6H10]
SKU/Catalog Number
M00109-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PDL1 CD274 Monoclonal Antibody [6H10] (Catalog # M00109-3). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PDL1 CD274 Monoclonal Antibody [6H10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109-3)
Host
Mouse
Contents
PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 6H10
Isotype
IgG1
Immunogen
Anti-PD-L1 antibody was raised against the extracellular domain of human PD-L1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PD-L1 Antibody has no cross-reactivity to PD-L2.
Reactive Species
M00109-3 is reactive to CD274 in Human, Mouse
Applications
M00109-3 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.275kDa
Background of PD-L1
PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3). Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.25 - 4 μg/mL; IF: 2 μg/mL; IHC-P: 5 μg/mL; ICC: 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry, Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Overexpression Validation of PD-L1 in 293 Cells
Loading: 15 μg of lysates per lane.Antibodies:M00109-3 (A, 0.25 μg/mL; B, 0.5 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: (2 μg/mL),M00109-3 (2 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit and or anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.
Click image to see more details
Validation with PD-L1 siRNA Knockdown in HeLa Cells
HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies:M00109-3 (2 μg/mL) and GAPDH (0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Click image to see more details
Western Blot Validation of PD-L1 in Raji Cells
Loading: Lysates/proteins at 15 μg per lane. Antibodies:M00109-3 (4 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunofluorescence Validation of PD-L1 in Transfected 293 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed PD-L1 transfected 293 cells labeling PD-L1 with M00109-3 at 2 μg/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Click image to see more details
Immunofluorescence Validation of PD-L1 in Human Stomach Carcinoma Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human stomach carcinoma tissue labeling PD-L1 with M00109-3 at 2 μg/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Click image to see more details
Immunofluorescence Validation of PD-L1 in Human Tonsil Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling PD-L1 with M00109-3 at 2 μg/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Click image to see more details
Immunohistochemistry Validation of PD-L1 in Human Stomach Carcinoma Tissue
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PD-L1 antibody (M00109-3) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Lower left: Use mouse IgG antibody at 1 μg/ml as control.
Click image to see more details
Immunohistochemistry Validation of PD-L1 in Human Tonsil Carcinoma Tissue
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-L1 antibody (M00109-3) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Lower left: Use mouse IgG antibody at 1 μg/ml as control.
Click image to see more details
Immunocytochemistry Validation of PD-L1 in PD-L1 Overexpressed 293 Cells
Immunocytochemical analysis of PD-L1 transfected 293 cells using anti-PD-L1 antibody (M00109-3) at 1 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Lower left: Use mouse IgG antibody at 1 μg/mL as control.
Protein Target Info & Infographic
Gene/Protein Information For CD274 (Source: Uniprot.org, NCBI)
Gene Name
CD274
Full Name
Programmed cell death 1 ligand 1
Weight
33.275kDa
Superfamily
immunoglobulin superfamily
Alternative Names
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 antigen|PDCD1 ligand 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD274, check out the CD274 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD274: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDL1 CD274 Monoclonal Antibody [6H10] (M00109-3)
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No publications found for M00109-3
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