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Product Info Summary
| SKU: | M01429-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Cow, Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC |
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Product info
Product Name
Anti-Perforin Purified PRF1 Monoclonal Antibody
SKU/Catalog Number
M01429-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Perforin Purified PRF1 Monoclonal Antibody (Catalog# M01429-1). Tested in Flow Cytometry, IP, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human, Cow.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Perforin Purified PRF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01429-1)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
dG9
Isotype
Mouse IgG2b kappa
Immunogen
purified granules from human YT lymphoma cell line. The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01429-1 is reactive to PRF1 in Cow, Human
Applications
M01429-1 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
61.377kDa
Background of Perforin
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 3-12 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For PRF1 (Source: Uniprot.org, NCBI)
Gene Name
PRF1
Full Name
Perforin-1
Weight
61.377kDa
Superfamily
complement C6/C7/C8/C9 family
Alternative Names
Cytolysin; FLH2; HPLH2; HPLH2lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; P1PFN1; perforin 1 (pore forming protein); Perforin; perforin-1; PFP; PFPcytolysin; PRF1 PRF1 HPLH2, P1, PFP perforin 1 perforin-1|cytolysin|lymphocyte pore-forming protein|perforin 1 (pore forming protein)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRF1, check out the PRF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Perforin Purified PRF1 Monoclonal Antibody (M01429-1)
Hello CJ!
M01429-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ferguson Bennit HR,Gonda A,Kabagwira J,Oppegard L,Chi D,Licero Campbell J,De Leon M,Wall NR.Natural Killer Cell Phenotype and Functionality Affected by Exposure to Extracellular Survivin and Lymphoma-Derived Exosomes.Int J Mol Sci.2021 Jan 27;22(3):1255.d
Species: Human
M01429-1 usage in article: APP:FLOW CYTOMETRY, SAMPLE:EXOSOMES, DILUTION:NA
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Customer Reviews
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