Product Info Summary
SKU: | P00026-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00026-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody catalog # P00026-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00026-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-c-Myc (T58 + S62)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00026-1 is reactive to MYC in Human, Rat
Applications
P00026-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
65 kDa
Calculated molecular weight
48.804kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:100-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phospho-c-Myc (T58 + S62) in HEK293 cell lysate treated with EGF.
Protein Target Info & Infographic
Gene/Protein Information For MYC (Source: Uniprot.org, NCBI)
Gene Name
MYC
Full Name
Myc proto-oncogene protein
Weight
48.804kDa
Alternative Names
9E10 Myc; 9E10 Myc; avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) MYC MRTLC, bHLHe39, c-Myc, MYC MYC proto-oncogene, bHLH transcription factor myc proto-oncogene protein|avian myelocytomatosis viral oncogene homolog|class E basic helix-loop-helix protein 39|myc-related translation/localization regulatory factor|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog|v-myc myelocytomatosis viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYC, check out the MYC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody (P00026-1)
Hello CJ!
P00026-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression
Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody
Question
We purchased anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody for WB on promyelocytic leukemia a few years ago. I am using rat, and We are going to use the antibody for IP next. I was wanting to use examining promyelocytic leukemia as well as cervix in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
I have checked the website and datasheets of our anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody and it seems that P00026-1 has been tested on rat in both WB and IP. Thus P00026-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-01
Question
We are currently using anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody P00026-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody (P00026-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
Question
We have been able to see staining in rat cervix. Are there any suggestions? Is anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody supposed to stain cervix positively?
L. Zhao
Verified customer
Asked: 2018-09-21
Answer
From what I have seen in literature cervix does express MYC. From what I have seen in Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-09-21
Question
Our team were well pleased with the WB result of your anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta testis nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
M. Edwards
Verified customer
Asked: 2015-09-30
Answer
Based on literature, placenta testis does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-09-30