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Product Info Summary
| SKU: | A02123S422 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-CtBP1 (S422) Antibody
SKU/Catalog Number
A02123S422
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-CtBP1 (S422) Antibody catalog # A02123S422. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-CtBP1 (S422) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02123S422)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CtBP1 around the phosphorylation site of S422.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02123S422 is reactive to CTBP1 in Human, Mouse, Rat
Applications
A02123S422 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
47.535kDa
Background of CTBP1
Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2) By similarity. Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage. Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth.
Hateboer G., EMBO J. 14:3159-3169(1995).
Deloukas P., Nature 429:375-381(2004).
Matsuoka S., Science 316:1160-1166(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of CTBP1 using Anti-Phospho-CtBP1 (S422) Antibody (A02123S422).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C
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Figure 1. Western blotting validation for Anti-Phospho-CtBP1 (S422) Antibody A02123S422
Western Blot (WB) analysis of JK cells using Phospho-CtBP1 (S422) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CTBP1 (Source: Uniprot.org, NCBI)
Gene Name
CTBP1
Full Name
C-terminal-binding protein 1
Weight
47.535kDa
Superfamily
D-isomer specific 2-hydroxyacid dehydrogenase family
Alternative Names
BARS; brefeldin A-ribosylated substrate; CTBP; CtBP1; C-terminal binding protein 1; C-terminal-binding protein 1; EC 1.1.1; EC 1.1.1.-; EC 1.1.1.158; MGC104684 CTBP1 BARS, HADDTS C-terminal binding protein 1 C-terminal-binding protein 1|brefeldin A-ribosylated substrate
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTBP1, check out the CTBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-CtBP1 (S422) Antibody (A02123S422)
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No publications found for A02123S422
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