Product Info Summary
SKU: | P02677-1 |
---|---|
Size: | 150 µl |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-GluR1 (Ser845) Gria1 Antibody
SKU/Catalog Number
P02677-1
Size
150 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-GluR1 (Ser845) Gria1 Antibody (Catalog # P02677-1). Tested in WB, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-GluR1 (Ser845) Gria1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02677-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
10C3
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 of rat GluR1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
P02677-1 is reactive to GRIA1 in Mouse, Rat
Applications
P02677-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
101.506kDa
Background of GluR1
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Protein Target Info & Infographic
Gene/Protein Information For GRIA1 (Source: Uniprot.org, NCBI)
Gene Name
GRIA1
Full Name
Glutamate receptor 1
Weight
101.506kDa
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR1; gluR-1; GLUR1gluR-A; GLURA; GluR-A; gluR-K1; glutamate receptor 1; Glutamate receptor ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA 1; GRIA1; HBGR1; MGC133252 GRIA1 GLUH1, GLUR1, GLURA, GluA1, HBGR1 glutamate ionotropic receptor AMPA type subunit 1 glutamate receptor 1|AMPA 1|AMPA-selective glutamate receptor 1|gluR-1|gluR-A|gluR-K1|glutamate receptor, ionotropic, AMPA 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIA1, check out the GRIA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-GluR1 (Ser845) Gria1 Antibody (P02677-1)
Hello CJ!
No publications found for P02677-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-GluR1 (Ser845) Gria1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Phospho-GluR1 (Ser845) Gria1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question