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- Table of Contents
ARHGAP35 antibody (P30433) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Anti-Phospho GRF-1 (Tyr1105) Antibody
ARHGAP35 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Phospho GRF-1 (Tyr1105) Antibody
View all ARHGAP35 Antibodies
View all ARHGAP35 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GRF-1 around the phosphorylation site of Tyr1105. AA range:1071-1120
Observed Molecular Weight
72 kDa
Calculated Molecular Weight
170.5 kDa
Cite This Product
Anti-Phospho GRF-1 (Tyr1105) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30433)
Review
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1 Review For Anti-Phospho GRF-1 (Tyr1105) Antibody
This antibody exhibits high specificity and clear bands, and the resulting experimental data are reliable, providing important support for elucidating the molecular mechanisms underlying the role of PTN in this study.
| SKU | P30433 |
|---|---|
| Application | Western Blot |
| Sample | Mouse Spinal |
| Sample Processing Description | Tissue samples were directly lysed in RIPA lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Then, 20 μl of each cell protein sample was loaded into each lane of the SDS-PAGE gel. |
| Primary Antibody | Phospho GRF-1 (Tyr1105) Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: Sangon Biotech |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |
Zhixiong Liu, Xiamen University
Verified customer
Submitted 2025-10-30
Product Promise
We promise Anti-Phospho GRF-1 (Tyr1105) Antibody performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P30433
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026