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Product Info Summary
| SKU: | P30433 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho GRF-1 (Tyr1105) Antibody
SKU/Catalog Number
P30433
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho GRF-1 (Tyr1105) Antibody (Catalog# P30433). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho GRF-1 (Tyr1105) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30433)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GRF-1 around the phosphorylation site of Tyr1105.
Reactive Species
P30433 is reactive to ARHGAP35 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
170.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30433 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of 3T3 cells using Phospho-GRF-1 (Y1105) Polyclonal Antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho GRF-1 (Tyr1105) Antibody (P30433)
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Customer Reviews
Have you used Anti-Phospho GRF-1 (Tyr1105) Antibody?
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1 Reviews For Anti-Phospho GRF-1 (Tyr1105) Antibody
This antibody exhibits high specificity and clear bands, and the resulting experimental data are reliable, providing important support for elucidating the molecular mechanisms underlying the role of PTN in this study.
Excellent
| SKU | P30433 |
|---|---|
| Application | Western Blot |
| Sample | Mouse Spinal |
| Sample Processing Description | Tissue samples were directly lysed in RIPA lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Then, 20 μl of each cell protein sample was loaded into each lane of the SDS-PAGE gel. |
| Primary Antibody | Phospho GRF-1 (Tyr1105) Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: Sangon Biotech |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |
Zhixiong Liu, Xiamen University
Verified customer
Submitted 2025-10-30
Customer Q&As
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7 Customer Q&As for Anti-Phospho GRF-1 (Tyr1105) Antibody
Question
Will anti-Phospho GRF-1 (Tyr1105) antibody P30433 work for WB with mammary cancer?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
According to the expression profile of mammary cancer, ARHGAP35 is highly expressed in mammary cancer. So, it is likely that anti-Phospho GRF-1 (Tyr1105) antibody P30433 will work for WB with mammary cancer.
Boster Scientific Support
Answered: 2020-04-10
Question
Is this P30433 anti-Phospho GRF-1 (Tyr1105) antibody reactive to the isotypes of ARHGAP35?
Z. Parker
Verified customer
Asked: 2019-08-01
Answer
The immunogen of P30433 anti-Phospho GRF-1 (Tyr1105) antibody is A peptide derived from human GRF-1 around the phosphorylation site of Tyr1105. Immunogen sequence location: 1071-1120. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary cancer using anti-Phospho GRF-1 (Tyr1105) antibody P30433. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-01
Question
We are currently using anti-Phospho GRF-1 (Tyr1105) antibody P30433 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
K. Williams
Verified customer
Asked: 2018-05-30
Answer
The anti-Phospho GRF-1 (Tyr1105) antibody (P30433) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-30
Question
We need to test anti-Phospho GRF-1 (Tyr1105) antibody P30433 on human mammary cancer for research purposes, then I may be interested in using anti-Phospho GRF-1 (Tyr1105) antibody P30433 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
The products we sell, including anti-Phospho GRF-1 (Tyr1105) antibody P30433, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-16
Question
Do you have a BSA free version of anti-Phospho GRF-1 (Tyr1105) antibody P30433 available?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho GRF-1 (Tyr1105) antibody P30433 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-15
Question
Can you help my question with product P30433, anti-Phospho GRF-1 (Tyr1105) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Taylor
Verified customer
Asked: 2014-08-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P30433 anti-Phospho GRF-1 (Tyr1105) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-08-19
