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Product Info Summary
| SKU: | P00157 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00157
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal Antibody catalog # P00157. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00157)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CHD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myb
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00157 is reactive to MYB in Human
Applications
P00157 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
72.341kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Validation Images & Assay Conditions
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Western blot analysis of Phospho-v-Myb + c-Myb (S11) Antibody expression in Ramos cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For MYB (Source: Uniprot.org, NCBI)
Gene Name
MYB
Full Name
Transcriptional activator Myb
Weight
72.341kDa
Alternative Names
c-myb protein (140 AA); Cmyb; c-myb; c-myb_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; efg; Proto-oncogene c-Myb; transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog; v-myb myeloblastosis viral oncogene homolog (avian) MYB Cmyb, c-myb, c-myb_CDS, efg MYB proto-oncogene, transcription factor transcriptional activator Myb|oncogene AMV|proto-oncogene c-Myb|v-myb avian myeloblastosis viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYB, check out the MYB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal Antibody (P00157)
Hello CJ!
No publications found for P00157
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oviduct epithelium using anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
Is a blocking peptide available for product anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody (P00157)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
We do provide the blocking peptide for product anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody (P00157). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-26
Question
I see that the anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-25
Question
We have seen staining in human oviduct epithelium. Any tips? Is anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody supposed to stain oviduct epithelium positively?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
From what I have seen in literature oviduct epithelium does express MYB. From what I have seen in Uniprot.org, MYB is expressed in oviduct epithelium, liver placenta, testis, erythroleukemia, among other tissues. Regarding which tissues have MYB expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Liver, and Placenta, Pubmed ID: 14574404
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-28
Question
Our lab were happy with the WB result of your anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody. However we have been able to see positive staining in liver placenta nucleus using this antibody. Is that expected? Could you tell me where is MYB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
From literature, liver placenta does express MYB. Generally MYB expresses in nucleus. Regarding which tissues have MYB expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Liver, and Placenta, Pubmed ID: 14574404
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-28
Question
We are interested in to test anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 on human oviduct epithelium for research purposes, then I may be interested in using anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The products we sell, including anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-14
Question
My question regarding product P00157, anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00157 anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-22
Question
Would P00157 anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
You can see on the product datasheet, P00157 anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-18
Question
We are currently using anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
R. Dhar
Verified customer
Asked: 2018-09-18
Answer
The anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody (P00157) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-18
Question
I was wanting to use your anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody for WB for human oviduct epithelium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oviduct epithelium identification?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
You can see on the product datasheet, P00157 anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human oviduct epithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-13
Question
Does anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 work for WB with oviduct epithelium?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
According to the expression profile of oviduct epithelium, MYB is highly expressed in oviduct epithelium. So, it is likely that anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 will work for WB with oviduct epithelium.
Boster Scientific Support
Answered: 2018-01-12
Question
I have attached the WB image, lot number and protocol we used for oviduct epithelium using anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-24
Question
Is this P00157 anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody reactive to the isotypes of MYB?
D. Lewis
Verified customer
Asked: 2016-08-04
Answer
The immunogen of P00157 anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody is A synthesized peptide derived from human Myb. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-08-04
Question
Do you have a BSA free version of anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 available?
S. Huang
Verified customer
Asked: 2014-04-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody P00157 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-04-21
Question
We ordered your anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody for IF on erythroleukemia a few years ago. I am using human, and We are going to use the antibody for WB next. Our lab want to know about examining erythroleukemia as well as liver placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
S. Edwards
Verified customer
Asked: 2014-01-29
Answer
I looked at the website and datasheets of our anti-Phospho-v-Myb + c-Myb (S11) Rabbit Monoclonal antibody and it seems that P00157 has been validated on human in both IF and WB. Thus P00157 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-01-29
