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Product Info Summary
| SKU: | A00653 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Pig, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody
SKU/Catalog Number
A00653
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody catalog # A00653. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Pig.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00653)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00653 is reactive to PRKACA in Human, Mouse, Pig, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
40.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00653 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western blot analysis of lysates from 1) 22RV1, 2) Hela , 3) COLO205 cells, (Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red） Tubulin β Monoclonal Antibody (5G3) antibody was diluted at 1:5000 as loading control, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PKA alpha/beta CAT Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of Hela cell. 1, PKAα/β/γ cat Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Specific Publications For Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody (A00653)
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Customer Q&As
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7 Customer Q&As for Anti-PKA alpha/ beta/ gamma cat PRKACA Antibody
Question
I see that the anti-PKA alpha/ beta/ gamma cat antibody A00653 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-24
Question
Is a blocking peptide available for product anti-PKA alpha/ beta/ gamma cat antibody (A00653)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We do provide the blocking peptide for product anti-PKA alpha/ beta/ gamma cat antibody (A00653). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-17
Question
We want to test anti-PKA alpha/ beta/ gamma cat antibody A00653 on rat testis for research purposes, then I may be interested in using anti-PKA alpha/ beta/ gamma cat antibody A00653 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The products we sell, including anti-PKA alpha/ beta/ gamma cat antibody A00653, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-PKA alpha/ beta/ gamma cat antibody A00653 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
The anti-PKA alpha/ beta/ gamma cat antibody (A00653) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-06
Question
Would A00653 anti-PKA alpha/ beta/ gamma cat antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
It shows on the product datasheet, A00653 anti-PKA alpha/ beta/ gamma cat antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-15
Question
I was wanting to use your anti-PKA alpha/ beta/ gamma cat antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
You can see on the product datasheet, A00653 anti-PKA alpha/ beta/ gamma cat antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-27
Question
See below the WB image, lot number and protocol we used for testis using anti-PKA alpha/ beta/ gamma cat antibody A00653. Please let me know if you require anything else.
L. Baker
Verified customer
Asked: 2016-01-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-13
