Product Info Summary
SKU: | PB9319 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-PKC beta 1/PRKCB Antibody Picoband™
SKU/Catalog Number
PB9319
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKC beta 1/PRKCB Antibody Picoband™ catalog # PB9319. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC beta 1/PRKCB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9319)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PKC beta 1 recombinant protein (Position: E542-V671). Human PKC beta 1 shares 100% amino acid (aa) sequence identity with both mouse and rat PKC beta 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9319 is reactive to PRKCB in Human, Mouse, Rat
Applications
PB9319 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
77 kDa
Calculated molecular weight
76.869kDa
Background of PRKCB
Protein kinase C beta type is an enzyme that in humans is encoded by the PRKCB gene. It is a member of the protein kinase C (PKC) gene family. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. It has been found that PRKCB activated by oxidative conditions in the cell, induces phosphorylation of p66 (SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (PB9319).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # PB9319) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC beta 1 at approximately 77 kDa. The expected band size for PKC beta 1 is at 77 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PRKCB (Source: Uniprot.org, NCBI)
Gene Name
PRKCB
Full Name
Protein kinase C beta type
Weight
76.869kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.13; MGC41878; PKC-B; PKC-beta; PKCBPRKCB2; PRKCB1protein kinase C beta 1; protein kinase C beta type; protein kinase C, beta 1 polypeptide; protein kinase C, beta 1; protein kinase C, beta PRKCB PKC-beta, PKCB, PKCI(2), PKCbeta1, PRKCB2, PRKCB protein kinase C beta protein kinase C beta type|PKC-B|protein kinase C, beta 1 polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCB, check out the PRKCB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC beta 1/PRKCB Antibody Picoband™ (PB9319)
Hello CJ!
PB9319 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors
Different localization and expression of protein kinase C-beta in kidney cortex of diabetic nephropathy mice and its role in telmisartan treatment
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-PKC beta 1/PRKCB Antibody Picoband™
Question
We are currently using anti-PKC beta 1/PRKCB antibody PB9319 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The anti-PKC beta 1/PRKCB antibody (PB9319) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-06