Anti-PKLR Antibody Picoband™

Product Name

Anti-PKLR Antibody Picoband™

View all PKLR Antibodies

SKU/Catalog Number

PB9499

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PKLR Antibody Picoband™ catalog # PB9499. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKLR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9499)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR, different from the related mouse sequence by one amino acid, and identical to the rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9499 is reactive to PKLR in Human, Mouse, Rat

Applications

PB9499 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

62 kDa

Calculated molecular weight

61.83kDa

Background of PKLR

Pyruvate kinase isozymes R/L is an enzyme that in humans is encoded by the PKLR gene. It is mapped to 1q21. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PKLR using anti-PKLR antibody (PB9499).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody (Catalog # PB9499) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKLR at approximately 62 kDa. The expected band size for PKLR is at 62 kDa.

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Figure 2. IHC analysis of PKLR using anti-PKLR antibody (PB9499).
PKLR was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PKLR Antibody (PB9499) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For PKLR (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.1.40; PK1; PK1PKRL; PKL; PKLR; PKR; Pyruvate Kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase isozymes R/L; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and RBC; Red cell/liver pyruvate kinase; RPK; R-type/L-type pyruvate kinase PKLR PK1, PKL, PKRL, RPK pyruvate kinase L/R pyruvate kinase PKLR|R-type/L-type pyruvate kinase|pyruvate kinase 1|pyruvate kinase isozyme R/L|pyruvate kinase isozymes L/R|pyruvate kinase isozymes R/L|pyruvate kinase type L|pyruvate kinase, liver and RBC|pyruvate kinase, liver and blood cell|red cell/liver pyruvate kinase

For more info on PKLR, check out the PKLR Infographic

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In this infographic, you will see the following information for PKLR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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