Anti-PKR/EIF2AK2 Antibody

Product Name

Anti-PKR/EIF2AK2 Antibody

View all PKR Antibodies

SKU/Catalog Number

PA2025

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PKR/EIF2AK2 Antibody catalog # PA2025. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKR/EIF2AK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2025)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PKR.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2025 is reactive to EIF2AK2 in Human

Applications

PA2025 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

94 kDa

Calculated molecular weight

62.094kDa

Background of PKR

EIF2AK2 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2), also called PKR, is an enzyme that in humans is encoded by the EIF2AK2 gene. Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis (summary by Kuhen et al., 1996). By FISH analysis, Squire et al. (1993) assigned the EIF2AK2 gene to the boundary between chromosome 2p22-p21. Ben-Asouli et al. (2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield. IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region. Taylor et al. (1999) studied the mechanism underlying the resistance of hepatitis C virus (HCV) to interferon. They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and the translation initiation factor EIF2-alpha, a target of PKR. E2 inhibited the kinase activity of PKR and blocked its inhibitory effect on protein synthesis and cell growth.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Anti-PKR antibody, PA2025, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK2, 35.0KD (162aa tag+ M1-K154)
Lane 1: Recombinant Human EIF2AK2 Protein 10ng
Lane 2: Recombinant Human EIF2AK2 Protein 5ng
Lane 3: Recombinant Human EIF2AK2 Protein 2.5ng
Lane 4: Recombinant Human EIF2AK2 Protein 1.25ng

Gene/Protein Information For EIF2AK2 (Source: Uniprot.org, NCBI)

Alternative Names

double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; P68 Kinase; PKR; PKRp68 kinase; PPP1R83; PRKR; PRKRMGC126524; Protein Kinase R; Protein kinase RNA-activated EIF2AK2 EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR eukaryotic translation initiation factor 2 alpha kinase 2 interferon-induced, double-stranded RNA-activated protein kinase|P1/eIF-2A protein kinase|double stranded RNA activated protein kinase|eIF-2A protein kinase 2|interferon-inducible elF2alpha kinase|p68 kinase|protein kinase R|protein kinase, interferon-inducible double stranded RNA dependent|protein phosphatase 1, regulatory subunit 83|tyrosine-protein kinase EIF2AK2

For more info on EIF2AK2, check out the EIF2AK2 Infographic

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In this infographic, you will see the following information for EIF2AK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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