Anti-Plasmolipin/PLLP Antibody Picoband™

Product Name

Anti-Plasmolipin/PLLP Antibody Picoband™

View all Plasmolipin Antibodies

SKU/Catalog Number

A10793

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Plasmolipin/PLLP Antibody Picoband™ catalog # A10793. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Plasmolipin/PLLP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10793)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Plasmolipin/PLLP recombinant protein (Position: M1-A142).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10793 is reactive to PLLP in Human

Applications

A10793 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

19.987kDa

Background of Plasmolipin

PLLP (Plasmolipin, also known as PMLP; TM4SF11), located on 16q13, is a Protein Coding gene. The gene produces a 19987 Da protein composed of 182 amino acids. Plasmolipin (PLLP) is a novel protein identified in human corneal epithelium and has been demonstrated to have a key role in epithelial cell differentiation in other tissues. Plasmolipin is a hydrophobic plasma membrane proteolipid present in both the kidney and brain. The mouse plasmolipin gene has the common organization of the 4-transmembrane (4TM) gene group, with 4 exons and a large first intron.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Plasmolipin/PLLP using anti-Plasmolipin/PLLP antibody (A10793).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plasmolipin/PLLP antigen affinity purified polyclonal antibody (Catalog # A10793) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plasmolipin/PLLP at approximately 26 kDa. The expected band size for Plasmolipin/PLLP is at 18,20 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Plasmolipin/PLLP antibody (A10793).
Overlay histogram showing HepG2 cells stained with A10793 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasmolipin/PLLP Antibody (A10793, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PLLP (Source: Uniprot.org, NCBI)

Alternative Names

Plasma membrane proteolipid; plasmolipin; PMLPTM4SF11plasma membrane proteolipid (plasmolipin); transmembrane 4 superfamily member 11 (plasmolipin) PLLP PMLP, TM4SF11 plasmolipin plasmolipin|plasma membrane proteolipid (plasmolipin)|transmembrane 4 superfamily member 11 (plasmolipin)

For more info on PLLP, check out the PLLP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PLLP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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