Product Info Summary
SKU: | M02965 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-PLAT/Tpa Rabbit Monoclonal Antibody
View all t-Plasminogen Activator/tPA Antibodies
SKU/Catalog Number
M02965
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PLAT/Tpa Rabbit Monoclonal Antibody catalog # M02965. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLAT/Tpa Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02965)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PLAT
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02965 is reactive to PLAT in Human, Mouse, Rat
Applications
M02965 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
28 kDa
Calculated molecular weight
62.917kDa
Background of t-Plasminogen Activator/tPA
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PLAT expression in human plasma lysate.
Protein Target Info & Infographic
Gene/Protein Information For PLAT (Source: Uniprot.org, NCBI)
Gene Name
PLAT
Full Name
Tissue-type plasminogen activator
Weight
62.917kDa
Superfamily
peptidase S1 family
Alternative Names
Alteplase; DKFZp686I03148; EC 3.4.21; EC 3.4.21.68; plasminogen activator, tissue type; plasminogen activator, tissue; PLAT; Reteplase; tissue plasminogen activator (t-PA); tissue-type plasminogen activator; TPA; T-PA; tPlasminogen Activator; t-Plasminogen Activator PLAT T-PA, TPA plasminogen activator, tissue type tissue-type plasminogen activator|alteplase|plasminogen/activator kringle|reteplase|t-plasminogen activator
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLAT, check out the PLAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLAT/Tpa Rabbit Monoclonal Antibody (M02965)
Hello CJ!
M02965 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel silkworm pupae carboxymethyl chitosan inhibits mouse L929 fibroblast proliferation. Lin Zhu,Zuo-Qing Fan,Xin-Qin Shi,Na Wang,Ying-Ying Bo,Hong-En Guo
Species: Silkworm,Mouse
M02965 usage in article: APP:WB, SAMPLE:FIBROBLASTS, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PLAT/Tpa Rabbit Monoclonal Antibody
Question
Is this M02965 anti-PLAT/Tpa Rabbit Monoclonal antibody reactive to the isotypes of PLAT?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The immunogen of M02965 anti-PLAT/Tpa Rabbit Monoclonal antibody is A synthesized peptide derived from human PLAT. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-30
Question
Would M02965 anti-PLAT/Tpa Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
As indicated on the product datasheet, M02965 anti-PLAT/Tpa Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-23
Question
I see that the anti-PLAT/Tpa Rabbit Monoclonal antibody M02965 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-05
Question
I was wanting to use your anti-PLAT/Tpa Rabbit Monoclonal antibody for WB for mouse fetal lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fetal lung identification?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
As indicated on the product datasheet, M02965 anti-PLAT/Tpa Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-06
Question
Does anti-PLAT/Tpa Rabbit Monoclonal antibody M02965 work on primate WB with placenta skin?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Our lab technicians have not tested anti-PLAT/Tpa Rabbit Monoclonal antibody M02965 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PLAT/Tpa Rabbit Monoclonal antibody M02965 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-07
Question
I have attached the WB image, lot number and protocol we used for fetal lung using anti-PLAT/Tpa Rabbit Monoclonal antibody M02965. Please let me know if you require anything else.
D. Wu
Verified customer
Asked: 2018-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-23