Anti-PLOD1 Antibody Picoband™

Product Name

Anti-PLOD1 Antibody Picoband™

View all PLOD1 Antibodies

SKU/Catalog Number

A05322-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PLOD1 Antibody Picoband™ catalog # A05322-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PLOD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05322-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human PLOD1 recombinant protein (Position: K23-A640).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05322-1 is reactive to PLOD1 in Human

Applications

A05322-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

84 kDa

Calculated molecular weight

83.55kDa

Background of PLOD1

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PLOD1 using anti-PLOD1 antibody (A05322-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLOD1 antigen affinity purified polyclonal antibody (Catalog # A05322-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLOD1 at approximately 84 kDa. The expected band size for PLOD1 is at 84 kDa.

Gene/Protein Information For PLOD1 (Source: Uniprot.org, NCBI)

Alternative Names

2-oxoglutarate 5-dioxygenase 1; EC 1.14.11.4; Ehlers-Danlos syndrome type VI); FLJ42041; LH1; LLH; lysine hydroxylase; Lysyl hydroxylase 1; PLODLH; procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase; procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1; procollagen-lysine; procollagen-lysine, 2-oxoglutarate 5-dioxygenase PLOD1 EDS6, EDSKCL1, LH, LH1, LLH, PLOD procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 procollagen-lysine,2-oxoglutarate 5-dioxygenase 1|lysine hydroxylase|lysyl hydroxlase 1|procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1

For more info on PLOD1, check out the PLOD1 Infographic

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In this infographic, you will see the following information for PLOD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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