Product Info Summary
SKU: | A13059 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-PPAPDC1B PLPP5 Antibody
View all PPAPDC1B/DPPL1 Antibodies
SKU/Catalog Number
A13059
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PPAPDC1B PLPP5 Antibody (Catalog # A13059). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
PPAPDC1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PPAPDC1B PLPP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13059)
Host
Rabbit
Contents
PPAPDC1B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
PPAPDC1B antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human PPAPDC1B. The immunogen is located within amino acids 30 - 80 of PPAPDC1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to other Rim proteins.
Reactive Species
A13059 is reactive to PLPP5 in Human, Mouse
Applications
A13059 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.484kDa
Background of PPAPDC1B/DPPL1
Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide (NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. Knockdown experiments indicated that this protein is involved with multiple cell signaling pathways, including the JAK-Stat3, MAP kinase, and PKC pathways. PPAPDC1B may also potentiate the estrogen receptor pathway by down-regulating DUSP22.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PPAPDC1B antibody can be used for detection of PPAPDC1B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PPAPDC1B in EL4 cell lysate with PPAPDC1B antibody at (A) 1 and (B) 2 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PLPP5 (Source: Uniprot.org, NCBI)
Gene Name
PLPP5
Full Name
Phospholipid phosphatase 5
Weight
29.484kDa
Superfamily
PA-phosphatase related phosphoesterase family
Alternative Names
diacylglycerol pyrophosphate like 1; DPPL1; EC 3.1.3.4; HTPAPphosphatidate phosphatase PPAPDC1B; phosphatidic acid phosphatase type 2 domain containing 1B; Phosphatidic acid phosphatase type 2 domain-containing protein 1B PLPP5 DPPL1, HTPAP, PPAPDC1B phospholipid phosphatase 5 phospholipid phosphatase 5|diacylglycerol pyrophosphate like 1|diacylglycerol pyrophosphate phosphatase-like 1|phosphatidate phosphatase PPAPDC1B|phosphatidic acid phosphatase type 2 domain containing 1B|phosphatidic acid phosphatase type 2 domain-containing protein 1B|testicular secretory protein Li 38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLPP5, check out the PLPP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLPP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPAPDC1B PLPP5 Antibody (A13059)
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No publications found for A13059
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