Product Info Summary
SKU: | M04060-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Protein LYRIC MTDH Monoclonal Antibody
SKU/Catalog Number
M04060-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Protein LYRIC MTDH Monoclonal Antibody catalog # M04060-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein LYRIC MTDH Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04060-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2F11C3
Isotype
IgG
Immunogen
Purified recombinant fragment of human MTDH expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M04060-1 is reactive to Mtdh in Human
Applications
M04060-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
63.846kDa
Background of LYRIC
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Flow Cytometry validation of MTDH using Anti-Protein LYRIC MTDH Monoclonal Antibody (M04060-1).
Flow cytometric (FCM) analysis of HeLa cells using MTDH Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 2. Immunohistochemistry validation of MTDH using Anti-Protein LYRIC MTDH Monoclonal Antibody (M04060-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver Tissues with AEC staining using MTDH Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Protein LYRIC MTDH Monoclonal Antibody M04060-1
Western Blot (WB) analysis using MTDH Monoclonal Antibody against K562 (1)Protein Target Info & Infographic
Gene/Protein Information For Mtdh (Source: Uniprot.org, NCBI)
Gene Name
Mtdh
Full Name
Protein LYRIC
Weight
63.846kDa
Alternative Names
3D3; AEG1; AEG-13D3/LYRIC; AEG1LYRIC/3D3; Astrocyte elevated gene-1 protein; LYRIC; LYRICMetastasis adhesion protein; Lysine-rich CEACAM1 co-isolated protein; Metadherin; MTDH; protein LYRIC Mtdh|2610103J23Rik, 3D3, 3D3/Ly, 3D3/Lyric, AEG-, AEG-1, AV353288, D8Bwg1112e, Ly, Lyric|metadherin|protein LYRIC|lysine-rich CEACAM1 co-isolated protein|metastasis adhesion protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mtdh, check out the Mtdh Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mtdh: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein LYRIC MTDH Monoclonal Antibody (M04060-1)
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No publications found for M04060-1
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Customer Reviews
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Customer Q&As
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