Anti-Rab5/RAB5A Antibody

RAB5A antibody

Boster Bio Anti-Rab5/RAB5A Antibody catalog # PA1773. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1773
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Rab5/RAB5A Antibody

View all RAB5A Antibodies

SKU/Catalog Number

PA1773

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Rab5/RAB5A Antibody catalog # PA1773. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rab5/RAB5A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1773)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Rab5 (138-155aa ANKRAVDFQEAQSYADDN), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1773 is reactive to RAB5A in Human, Mouse, Rat

Applications

PA1773 is guaranteed for IHC, WB Boster Guarantee

Background of RAB5A

RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For RAB5A (Source: Uniprot.org, NCBI)

Uniprot ID

P20339

Gene ID

5868

Gene Name

RAB5A

Full Name

Ras-related protein Rab-5A

Weight

23.659kDa

Superfamily

small GTPase superfamily

Alternative Names

RAB5A, member RAS oncogene family; RAB5RAS-associated protein RAB5A; ras-related protein Rab-5A RAB5A RAB5 RAB5A, member RAS oncogene family ras-related protein Rab-5A|RAS-associated protein RAB5A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAB5A, check out the RAB5A Infographic

RAB5A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAB5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1773 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Han F, Jiang J, Ding J, Liu H, Xiao B, Shi Y. Front Behav Neurosci. 2017 Apr 26;11:62. doi: 10.3389/fnbeh.2017.00062. eCollection 2017. Change of Rin1 and Stathmin in the Animal Model of Traumatic Stresses

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16 Customer Q&As for Anti-Rab5/RAB5A Antibody

Question

Is a blocking peptide available for product anti-Rab5/RAB5A antibody (PA1773)?

S. Kulkarni

Verified customer

Asked: 2020-05-01

Answer

We do provide the blocking peptide for product anti-Rab5/RAB5A antibody (PA1773). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-05-01

Question

I see that the anti-Rab5/RAB5A antibody PA1773 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-03

Question

Is this PA1773 anti-Rab5/RAB5A antibody reactive to the isotypes of RAB5A?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

The immunogen of PA1773 anti-Rab5/RAB5A antibody is A synthetic peptide corresponding to a sequence in the middle region of human Rab5(138-155aa ANKRAVDFQEAQSYADDN), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-02

Question

My team were happy with the WB result of your anti-Rab5/RAB5A antibody. However we have observed positive staining in substantia nigra cell membrane using this antibody. Is that expected? Could you tell me where is RAB5A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

Based on literature, substantia nigra does express RAB5A. Generally RAB5A expresses in cell membrane. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2020-02-21

Question

See below the WB image, lot number and protocol we used for cervix carcinoma using anti-Rab5/RAB5A antibody PA1773. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-20

Question

I would like to test anti-Rab5/RAB5A antibody PA1773 on rat cervix carcinoma for research purposes, then I may be interested in using anti-Rab5/RAB5A antibody PA1773 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

The products we sell, including anti-Rab5/RAB5A antibody PA1773, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-03

Question

We bought anti-Rab5/RAB5A antibody for WB on cervix carcinoma a few months ago. I am using rat, and I plan to use the antibody for IHC next. We need examining cervix carcinoma as well as cervix placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

I have checked the website and datasheets of our anti-Rab5/RAB5A antibody and it seems that PA1773 has been tested on rat in both WB and IHC. Thus PA1773 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-05

Question

Does anti-Rab5/RAB5A antibody PA1773 work for IHC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

According to the expression profile of cervix carcinoma, RAB5A is highly expressed in cervix carcinoma. So, it is likely that anti-Rab5/RAB5A antibody PA1773 will work for IHC with cervix carcinoma.

Boster Scientific Support

Answered: 2019-10-07

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Rab5/RAB5A antibody PA1773. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-12

Question

Do you have a BSA free version of anti-Rab5/RAB5A antibody PA1773 available?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab5/RAB5A antibody PA1773 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-26

Question

We have been able to see staining in mouse brain subthalamic nucleus. Any tips? Is anti-Rab5/RAB5A antibody supposed to stain brain subthalamic nucleus positively?

Verified Customer

Verified customer

Asked: 2019-03-20

Answer

From what I have seen in literature brain subthalamic nucleus does express RAB5A. From what I have seen in Uniprot.org, RAB5A is expressed in substantia nigra, brain, brain subthalamic nucleus, cervix placenta, cervix carcinoma, melanoma, among other tissues. Regarding which tissues have RAB5A expression, here are a few articles citing expression in various tissues:
Brain, and Subthalamic nucleus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 11062261, 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2019-03-20

Question

I am interested in using your anti-Rab5/RAB5A antibody for receptor internalization involved in canonical wnt signaling pathway studies. Has this antibody been tested with western blotting on rat ovary tissue? We would like to see some validation images before ordering.

J. Taylor

Verified customer

Asked: 2018-07-12

Answer

I appreciate your inquiry. This PA1773 anti-Rab5/RAB5A antibody is validated on rat ovary tissue, brain tissue, tissue lysate, human placenta tissue, hela cell lysate, 293t cell lysate, colo320 cell lysate, mm453 cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-07-12

Question

We are currently using anti-Rab5/RAB5A antibody PA1773 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-16

Answer

The anti-Rab5/RAB5A antibody (PA1773) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-16

Question

I was wanting to use your anti-Rab5/RAB5A antibody for IHC for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2017-06-26

Answer

You can see on the product datasheet, PA1773 anti-Rab5/RAB5A antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-26

Question

Can you help my question with product PA1773, anti-Rab5/RAB5A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Mitchell

Verified customer

Asked: 2017-06-12

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1773 anti-Rab5/RAB5A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-06-12

Question

Would PA1773 anti-Rab5/RAB5A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

O. Yang

Verified customer

Asked: 2013-05-06

Answer

As indicated on the product datasheet, PA1773 anti-Rab5/RAB5A antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-05-06

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Total: $315

SKU:PA1773

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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