|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Rab5 Antibody|
|Description||Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A(RAB5A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Rab5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1773)|
|Replacement Item||This antibody may replace the following items: sc-130010|sc-166600|sc-166601|sc-166622|sc-26566|sc-28570|sc-309|sc-46692 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Rab5(138-155aa ANKRAVDFQEAQSYADDN), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Ras-related protein Rab-5A|
|Molecular Weight||23659 MW|
|Protein Function||The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. .|
|Subcellular Localization||Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome. Cytoplasmic vesicle . Cell projection, ruffle . Membrane. Cytoplasm, cytosol. Enriched in stage I melanosomes. Alternates between membrane-bound and cytosolic forms.|
|Alternative Names||Ras-related protein Rab-5A;RAB5A;RAB5;|
|Research Areas|||neuroscience|neurotransmission|secretory vesicles|rabs| tags & cell markers|subcellular markers|organelles|endosome| signal transduction|protein trafficking|vesicle transport|regulation| neuroscience|cell type marker|neuron marker|synapse marker||
Background for Ras-related protein Rab-5A
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Rab5 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Rab5 Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Ovary Tissue Lysate
Lane 3: Human Placenta Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A375 Cell Lysate
Lane 7: COLO320 Cell Lysate
Lane 8: MM453 Cell Lysate
IHC(P):Rat Brain Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,