Product Info Summary
SKU: | A06262 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RAP80 UIMC1 Antibody
SKU/Catalog Number
A06262
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RAP80 UIMC1 Antibody (Catalog # A06262). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
RAP80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RAP80 UIMC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06262)
Host
Rabbit
Contents
RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RAP80. The immunogen is located within amino acids 520 - 570 of RAP80.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.
Reactive Species
A06262 is reactive to Uimc1 in Human, Mouse
Applications
A06262 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
81.478kDa
Background of RAP80
RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RAP80 antibody can be used for detection of RAP80 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 2 μg/mL.
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Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 μg/mL.
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Immunofluorescence of RAP80 in Human Spleen cells with RAP80 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Uimc1 (Source: Uniprot.org, NCBI)
Gene Name
Uimc1
Full Name
BRCA1-A complex subunit RAP80
Weight
81.478kDa
Superfamily
RAP80 family
Alternative Names
BRCA1-A complex subunit RAP80; RAP80; RAP80X2HRIP110; receptor associated protein 80; Receptor-associated protein 80; retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RXRIP110; ubiquitin interaction motif containing 1; Ubiquitin interaction motif-containing protein 1; UIMC1; X2HRIP110 Uimc1|9430016E08Rik, D330018D10Rik, D630032M02Rik, RIP110, Rxri, Rxrip110|ubiquitin interaction motif containing 1|BRCA1-A complex subunit RAP80|receptor-associated protein 80|retinoid X receptor interacting protein 110|ubiquitin interaction motif-containing protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Uimc1, check out the Uimc1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Uimc1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAP80 UIMC1 Antibody (A06262)
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