Anti-REST Antibody

Product Name

Anti-REST Antibody

View all NRSF Antibodies

SKU/Catalog Number

A03852

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-REST Antibody (Catalog # A03852). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-REST Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03852)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.873~877(R-E-E-A-S) derived from Human REST.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03852 is reactive to REST in Human

Applications

A03852 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

121.872kDa

Background of NRSF

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

Chong J.A.et.al. (1995)Cell 80:949-957
Schoenherr C.J.et.al.(1995)Science 267:1360-1363
Scholl T.et.al.(1996)J. Immunol. 156:1448-1457
Lunyak V.V.et.al. (2002)Science 298:1747-1752

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 121kd
Western blotting: 1:500~1:1000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of REST using anti-REST antibody (A03852).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REST antigen affinity purified polyclonal antibody (Catalog # A03852) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for REST.

Gene/Protein Information For REST (Source: Uniprot.org, NCBI)

Alternative Names

Neural-restrictive silencer factor; NRSFneuron restrictive silencer factor; RE1-silencing transcription factor; X2 box repressor; XBRrepressor binding to the X2 box REST DFNA27, GINGF5, HGF5, NRSF, WT6, XBR RE1 silencing transcription factor RE1-silencing transcription factor|neural-restrictive silencer factor|neuron restrictive silencer factor|repressor binding to the X2 box

For more info on REST, check out the REST Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for REST: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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