Product Info Summary
SKU: | A00141-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RIPK1 Antibody
View all RIPK1/RIP1 Antibodies
SKU/Catalog Number
A00141-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RIPK1 Antibody (Catalog # A00141-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RIPK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RIPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00141-1)
Host
Rabbit
Contents
RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RIPK1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1. The immunogen is located within amino acids 180 - 230 of RIPK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.
Reactive Species
A00141-1 is reactive to RIPK1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
75931 MW
Background of RIPK1/RIP1
RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NFκ-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-κB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00141-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RIPK1 antibody can be used for detection of RIPK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RIPK1 in rat kidney tissue lysate with RIPK1 antibody at 1 μg/mL.
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Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/mL.
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Immunofluorescence of RIPK1 in Mouse Kidney cells with RIPK1 antibody at 20 μg/mL.
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Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For RIPK1 (Source: Uniprot.org, NCBI)
Gene Name
RIPK1
Full Name
Receptor-interacting serine/threonine-protein kinase 1
Weight
75931 MW
Superfamily
protein kinase superfamily
Alternative Names
RIP, RIP1, RIP, Cell death protein RIP, RIP-1 RIPK1 AIEFL, IMD57, RIP, RIP-1, RIP1 receptor interacting serine/threonine kinase 1 receptor-interacting serine/threonine-protein kinase 1|cell death protein RIP|receptor (TNFRSF)-interacting serine-threonine kinase 1|receptor-interacting protein 1|receptor-interacting protein kinase 1|serine/threonine-protein kinase RIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIPK1, check out the RIPK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIPK1 Antibody (A00141-1)
Hello CJ!
A00141-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen H,Tang LJ,Tu H,Zhou YJ,Li NS,Luo XJ,Peng J.Arctiin protects rat heart against ischemia/reperfusion injury via a mechanism involving reduction of necroptosis.Eur J Pharmacol.2020 May 15;875:173053.doi:10.1016/j.ejphar.2020.173053.Epub 2020 Mar 2.PMID:32135123.
Species: Rat
A00141-1 usage in article: APP:WB, SAMPLE:MYOCARDIAL TISSUE AND H9C2 CELL, DILUTION:NA
Tu H,Zhou YJ,Tang LJ,Xiong XM,Zhang XJ,Ali Sheikh MS,Zhang JJ,Luo XJ,Yuan C,Peng J. Combination of ponatinib with deferoxamine synergistically mitigates ischemic heart injury via simultaneous prevention of necroptosis and ferroptosis. Eur J Pharmacol.2021 Mar 3;898:173999.doi:10.1016/j.ejphar.2021.173999.Epub ahead of print.PMID:33675785.
Species: Rat
A00141-1 usage in article: APP:WB, SAMPLE:CARDIAC TISSUE AND H9C2 CELL, DILUTION:NA
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