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- Table of Contents
ROCK1 antibody (M00722) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Immunofluorescent analysis of Hela cells, using ROCK1 Antibody .
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Anti-ROCK1 Rabbit Monoclonal Antibody
ROCK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
ROCK1 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human U2OS whole cell, human 293T whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ROCK1 antibody was positively detected by IHC in:
human kidney tissue, Rat stomach tissue, Rat heart tissue, Human squamous carcinoma tissue, Human esophageal carcinoma tissue, Mouse heart tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-ROCK1 Rabbit Monoclonal Antibody
View all ROCK1 Antibodies
View all ROCK1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CDE-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ROCK1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
170 kDa
Calculated Molecular Weight
158.2 kDa
Cite This Product
Anti-ROCK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00722)
Protocols
Western Blot protocol for ROCK1 antibody
Immunohistochemistry (IHC) protocol for ROCK1 antibody
Publications
Application:
Species:
Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway
Published: 2024-01-11
Journal: CHEMISTRY & BIODIVERSITY
Application: WB
Impact Factor: 2.30
Species: Rat
PMID: 38212902
High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway
Published: 2022-07-08
Journal: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Application: WB
Impact Factor: 4.93
Species: Mouse
PMID: 35881407
Dietary Calcium Alleviates Fluorine-Induced Liver Injury in Rats by Mitochondrial Apoptosis Pathway
Published: 2021-02-24
Journal: BIOLOGICAL TRACE ELEMENT RESEARCH
Application: IHC
Impact Factor: 3.74
Species: Rat
PMID: 33629228
Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction
Published: 2019-04-17
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 3.74
Species: Human
PMID: 31004838
Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling
Published: 2018-05-27
Journal: MEDICAL SCIENCE MONITOR
Application: WB,IHC
Impact Factor: 1.89
Species: Human
PMID: 29804125
β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice
Published: 2014-01-20
Journal: BRAIN RESEARCH
Application: IHC,WB
Impact Factor: 2.83
Species: Mouse
PMID: 24457043
ROCK pathway participates in the processes that 15-hydroxyeicosatetraenoic acid (15-HETE) mediated the pulmonary vascular remodeling induced by hypoxia in rat
Published: 2009-09-10
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.31
Species: Rat
PMID: 19746421
Review
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Product Promise
We promise Anti-ROCK1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00722
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026