Anti-ROCK1 Rabbit Monoclonal Antibody

Western blot analysis of FROCK1 using anti-ROCK1 antibody (M00722).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified monoclonal antibody (Catalog # M00722) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 170 kDa. The expected band size for ROCK1 is at 158 kDa.

Western blot analysis of ROCK1 using anti-ROCK1 antibody (M00722).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549- WT whole cell lysates,
Lane 2: human A549-ROCK1 KO whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified monoclonal antibody (M00722) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 158 kDa. The expected band size for ROCK1 is at 158 kDa.

Immunohistochemical analysis of paraffin-embedded human kidney, using ROCK1 Antibody(M00722)
ROCK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROCK1 Antibody (M00722)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:150 dilution.

Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:150 dilution.

Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:150 dilution.

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:150 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:150 dilution.

Immunofluorescent analysis of Hela cells, using ROCK1 Antibody .

Immunofluorescent analysis using the Antibody at 1:50 dilution.